Chromatin state analysis of the barley epigenome reveals a higher-order structure defined by H3K27me1 and H3K27me3 abundance

Katie Baker, Taniya Dhillon, Isabelle Colas, Nicola Cook, Iain Milne, Linda Milne, Micha Bayer, Andrew J. Flavell (Lead / Corresponding author)

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Abstract

Combinations of histones carrying different covalent modifications are a major component of epigenetic variation. We have mapped nine modified histones in the barley seedling epigenome by chromatin immunoprecipitation next-generation sequencing (ChIP-seq). The chromosomal distributions of the modifications group them into four different classes, and members of a given class also tend to coincide at the local DNA level, suggesting that global distribution patterns reflect local epigenetic environments. We used this peak sharing to define 10 chromatin states representing local epigenetic environments in the barley genome. Five states map mainly to genes and five to intergenic regions. Two genic states involving H3K36me3 are preferentially associated with constitutive gene expression, while an H3K27me3-containing genic state is associated with differentially expressed genes. The 10 states display striking distribution patterns that divide barley chromosomes into three distinct global environments. First, telomere-proximal regions contain high densities of H3K27me3 covering both genes and intergenic DNA, together with very low levels of the repressive H3K27me1 modification. Flanking these are gene-rich interior regions that are rich in active chromatin states and have greatly decreased levels of H3K27me3 and increasing amounts of H3K27me1 and H3K9me2. Lastly, H3K27me3-depleted pericentromeric regions contain gene islands with active chromatin states separated by extensive retrotransposon-rich regions that are associated with abundant H3K27me1 and H3K9me2 modifications. We propose an epigenomic framework for barley whereby intergenic H3K27me3 specifies facultative heterochromatin in the telomere-proximal regions and H3K27me1 is diagnostic for constitutive heterochromatin elsewhere in the barley genome.

Original languageEnglish
Pages (from-to)111-124
Number of pages14
JournalPlant Journal
Volume84
Issue number1
Early online date8 Aug 2015
DOIs
Publication statusPublished - Oct 2015

Fingerprint

Hordeum
Chromatin
chromatin
Epigenomics
epigenetics
barley
Intergenic DNA
Heterochromatin
Genes
telomeres
Telomere
heterochromatin
intergenic DNA
genes
histones
Histones
Genome
Retroelements
genome
Chromatin Immunoprecipitation

Keywords

  • Barley
  • Chromatin immunoprecipitation next-generation sequencing
  • Epigenomics
  • Heterochromatin
  • Histone modification
  • Hordeum vulgare
  • Pericentromeric
  • PRJEB8068

Cite this

Baker, K., Dhillon, T., Colas, I., Cook, N., Milne, I., Milne, L., ... Flavell, A. J. (2015). Chromatin state analysis of the barley epigenome reveals a higher-order structure defined by H3K27me1 and H3K27me3 abundance. Plant Journal, 84(1), 111-124. https://doi.org/10.1111/tpj.12963
Baker, Katie ; Dhillon, Taniya ; Colas, Isabelle ; Cook, Nicola ; Milne, Iain ; Milne, Linda ; Bayer, Micha ; Flavell, Andrew J. / Chromatin state analysis of the barley epigenome reveals a higher-order structure defined by H3K27me1 and H3K27me3 abundance. In: Plant Journal. 2015 ; Vol. 84, No. 1. pp. 111-124.
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Baker, K, Dhillon, T, Colas, I, Cook, N, Milne, I, Milne, L, Bayer, M & Flavell, AJ 2015, 'Chromatin state analysis of the barley epigenome reveals a higher-order structure defined by H3K27me1 and H3K27me3 abundance', Plant Journal, vol. 84, no. 1, pp. 111-124. https://doi.org/10.1111/tpj.12963

Chromatin state analysis of the barley epigenome reveals a higher-order structure defined by H3K27me1 and H3K27me3 abundance. / Baker, Katie; Dhillon, Taniya; Colas, Isabelle; Cook, Nicola; Milne, Iain; Milne, Linda; Bayer, Micha; Flavell, Andrew J. (Lead / Corresponding author).

In: Plant Journal, Vol. 84, No. 1, 10.2015, p. 111-124.

Research output: Contribution to journalArticle

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T1 - Chromatin state analysis of the barley epigenome reveals a higher-order structure defined by H3K27me1 and H3K27me3 abundance

AU - Baker, Katie

AU - Dhillon, Taniya

AU - Colas, Isabelle

AU - Cook, Nicola

AU - Milne, Iain

AU - Milne, Linda

AU - Bayer, Micha

AU - Flavell, Andrew J.

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AB - Combinations of histones carrying different covalent modifications are a major component of epigenetic variation. We have mapped nine modified histones in the barley seedling epigenome by chromatin immunoprecipitation next-generation sequencing (ChIP-seq). The chromosomal distributions of the modifications group them into four different classes, and members of a given class also tend to coincide at the local DNA level, suggesting that global distribution patterns reflect local epigenetic environments. We used this peak sharing to define 10 chromatin states representing local epigenetic environments in the barley genome. Five states map mainly to genes and five to intergenic regions. Two genic states involving H3K36me3 are preferentially associated with constitutive gene expression, while an H3K27me3-containing genic state is associated with differentially expressed genes. The 10 states display striking distribution patterns that divide barley chromosomes into three distinct global environments. First, telomere-proximal regions contain high densities of H3K27me3 covering both genes and intergenic DNA, together with very low levels of the repressive H3K27me1 modification. Flanking these are gene-rich interior regions that are rich in active chromatin states and have greatly decreased levels of H3K27me3 and increasing amounts of H3K27me1 and H3K9me2. Lastly, H3K27me3-depleted pericentromeric regions contain gene islands with active chromatin states separated by extensive retrotransposon-rich regions that are associated with abundant H3K27me1 and H3K9me2 modifications. We propose an epigenomic framework for barley whereby intergenic H3K27me3 specifies facultative heterochromatin in the telomere-proximal regions and H3K27me1 is diagnostic for constitutive heterochromatin elsewhere in the barley genome.

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KW - Histone modification

KW - Hordeum vulgare

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KW - PRJEB8068

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