Chromosome Duplication in Saccharomyces cerevisiae

Stephen P. Bell, Karim Labib

Research output: Contribution to journalArticlepeer-review

238 Citations (Scopus)
250 Downloads (Pure)


The accurate and complete replication of genomic DNA is essential for all life. In eukaryotic cells, the assembly of the multi-enzyme replisomes that perform replication is divided into stages that occur at distinct phases of the cell cycle. Replicative DNA helicases are loaded around origins of DNA replication exclusively during G1 phase. The loaded helicases are then activated during S phase and associate with the replicative DNA polymerases and other accessory proteins. The function of the resulting replisomes is monitored by checkpoint proteins that protect arrested replisomes and inhibit new initiation when replication is inhibited. The replisome also coordinates nucleosome disassembly and assembly and the establishment of sister chromatid cohesion. Finally, when two replisomes converge they are disassembled. Studies in S. cerevisiae have led the way in our understanding of these processes. Here, we review our increasingly molecular understanding of these events and their regulation.
Original languageEnglish
Pages (from-to)1027-1067
Number of pages41
Issue number3
Publication statusPublished - 6 Jul 2016


  • DNA replication
  • cell cycle
  • chromatin
  • chromosome duplication
  • genome stability
  • YeastBook


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