Circulating tissue factor-positive procoagulant microparticles in patients with type 1 diabetes

Chenghui Zhang, Qing Ou, Yan Gu, Gaiping Cheng, Rong Du, Li Yuan, Ruth Cordiner, Deying Kang, Jiaying Zhang, Qiaorong Huang, Chuan Yu, Li Kang, Xuan Wang, Xin Sun, Xianming Mo, Haoming Tian, Ewan Pearson, Wentong Meng (Lead / Corresponding author), Sheyu Li (Lead / Corresponding author)

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Abstract

Aim: To investigate the count of circulating tissue factor-positive (TF +) procoagulant microparticles (MPs) in patients with type 1 diabetes mellitus (T1DM).

Methods: This case-control study included patients with T1DM and age and sex-matched healthy volunteers. The counts of phosphatidylserine-positive (PS +) MPs and TF +PS +MPs and the subgroups derived from different cell types were measured in the peripheral blood sample of the two groups using multicolor flow cytometric assay. We compared the counts of each MP between groups as well as the ratio of the TF +PS +MPs and PS +MPs (TF +PS +MPs/PS +MPs).

Results: We recruited 36 patients with T1DM and 36 matched healthy controls. Compared with healthy volunteers, PS +MPs, TF +PS +MPs and TF +PS +MPs/PS +MPs were elevated in patients with T1DM (PS +MPs: 1078.5 ± 158.08 vs 686.84 ± 122.04/μL, P <0.001; TF + PS +MPs: 202.10 ± 47.47 vs 108.33 ± 29.42/μL, P <0.001; and TF +PS +MPs/PS +MPs: 0.16 ± 0.04 vs 0.19 ± 0.05, P = 0.004), mostly derived from platelet, lymphocytes and endothelial cells. In the subgroup analysis, the counts of total and platelet TF +PS +MPs were increased in patients with diabetic retinopathy (DR) and with higher HbA1c, respectively.

Conclusion: Circulating TF +PS +MPs and those derived from platelet, lymphocytes and endothelial cells were elevated in patients with T1DM.

Original languageEnglish
Pages (from-to)2819-2828
Number of pages10
JournalDiabetes, Metabolic Syndrome and Obesity: Targets and Therapy
Volume12
DOIs
Publication statusPublished - 3 Jan 2020

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Keywords

  • Diabetic retinopathy
  • Microparticles
  • Tissue factor
  • Type 1 diabetes mellitus
  • type 1 diabetes mellitus
  • tissue factor
  • microparticles
  • diabetic retinopathy

Cite this

Zhang, C., Ou, Q., Gu, Y., Cheng, G., Du, R., Yuan, L., Cordiner, R., Kang, D., Zhang, J., Huang, Q., Yu, C., Kang, L., Wang, X., Sun, X., Mo, X., Tian, H., Pearson, E., Meng, W., & Li, S. (2020). Circulating tissue factor-positive procoagulant microparticles in patients with type 1 diabetes. Diabetes, Metabolic Syndrome and Obesity: Targets and Therapy, 12, 2819-2828. https://doi.org/10.2147/DMSO.S225761