Classification of the nucleolytic ribozymes based upon catalytic mechanism

David M. J. Lilley (Lead / Corresponding author)

Research output: Contribution to journalReview article

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Abstract

The nucleolytic ribozymes carry out site-specific RNA cleavage reactions by nucleophilic attack of the 2'-oxygen atom on the adjacent phosphorus with an acceleration of a million-fold or greater. A major part of this arises from concerted general acid-base catalysis. Recent identification of new ribozymes has expanded the group to a total of nine and this provides a new opportunity to identify sub-groupings according to the nature of the general base and acid. These include nucleobases, hydrated metal ions, and 2'-hydroxyl groups. Evolution has selected a number of different combinations of these elements that lead to efficient catalysis. These differences provide a new mechanistic basis for classifying these ribozymes.

Original languageEnglish
Article number1462
Pages (from-to)1-10
Number of pages10
JournalF1000Research
Volume8
DOIs
Publication statusPublished - 19 Aug 2019

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Catalytic RNA
Catalysis
RNA Cleavage
Acids
Hydroxyl Radical
Phosphorus
Metal ions
Metals
RNA
Oxygen
Atoms

Keywords

  • Nucleolytic ribozymes
  • general acid-base catalysis
  • metal ions

Cite this

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abstract = "The nucleolytic ribozymes carry out site-specific RNA cleavage reactions by nucleophilic attack of the 2'-oxygen atom on the adjacent phosphorus with an acceleration of a million-fold or greater. A major part of this arises from concerted general acid-base catalysis. Recent identification of new ribozymes has expanded the group to a total of nine and this provides a new opportunity to identify sub-groupings according to the nature of the general base and acid. These include nucleobases, hydrated metal ions, and 2'-hydroxyl groups. Evolution has selected a number of different combinations of these elements that lead to efficient catalysis. These differences provide a new mechanistic basis for classifying these ribozymes.",
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Classification of the nucleolytic ribozymes based upon catalytic mechanism. / Lilley, David M. J. (Lead / Corresponding author).

In: F1000Research, Vol. 8, 1462, 19.08.2019, p. 1-10.

Research output: Contribution to journalReview article

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AB - The nucleolytic ribozymes carry out site-specific RNA cleavage reactions by nucleophilic attack of the 2'-oxygen atom on the adjacent phosphorus with an acceleration of a million-fold or greater. A major part of this arises from concerted general acid-base catalysis. Recent identification of new ribozymes has expanded the group to a total of nine and this provides a new opportunity to identify sub-groupings according to the nature of the general base and acid. These include nucleobases, hydrated metal ions, and 2'-hydroxyl groups. Evolution has selected a number of different combinations of these elements that lead to efficient catalysis. These differences provide a new mechanistic basis for classifying these ribozymes.

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