Projects per year
Abstract
The nucleolytic ribozymes carry out site-specific RNA cleavage reactions by nucleophilic attack of the 2'-oxygen atom on the adjacent phosphorus with an acceleration of a million-fold or greater. A major part of this arises from concerted general acid-base catalysis. Recent identification of new ribozymes has expanded the group to a total of nine and this provides a new opportunity to identify sub-groupings according to the nature of the general base and acid. These include nucleobases, hydrated metal ions, and 2'-hydroxyl groups. Evolution has selected a number of different combinations of these elements that lead to efficient catalysis. These differences provide a new mechanistic basis for classifying these ribozymes.
Original language | English |
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Article number | 1462 |
Number of pages | 10 |
Journal | F1000Research |
Volume | 8 |
DOIs | |
Publication status | Published - 19 Aug 2019 |
Keywords
- Nucleolytic ribozymes
- general acid-base catalysis
- metal ions
ASJC Scopus subject areas
- General Biochemistry,Genetics and Molecular Biology
- General Immunology and Microbiology
- Pharmacology, Toxicology and Pharmaceutics(all)
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Dive into the research topics of 'Classification of the nucleolytic ribozymes based upon catalytic mechanism'. Together they form a unique fingerprint.Projects
- 3 Finished
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Dynamics of Eukaryotic Junction-Resolving Enzyme GEN1 - DNA Junction Interactions
Lilley, D. (Investigator)
Biotechnology and Biological Sciences Research Council
1/10/16 → 30/09/19
Project: Research
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The Nucleic Acid Structure Research Group
Lilley, D. (Investigator)
1/01/16 → 31/12/22
Project: Research
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Fluorescence Resonance Energy Transfer as a Rich Source of Orientational Information in Nucleic Acid Structure
Lilley, D. (Investigator)
Engineering and Physical Sciences Research Council
1/09/12 → 30/06/16
Project: Research