Cloning and characterization of a U6 small nuclear RNA gene from potato

Yunqian Hu, John W.S. Brown, Robbie Waugh, Philip C. Turner (Lead / Corresponding author)

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Using a mixed U6 snRNA gene probe and a low stringency hybridization procedure we have isolated a U6 snRNA containing clone from a potato genomic library in λ EMBL 3. This clone cotains a single U6 snRNA gene which has been subcloned and sequenced. Southern blotting experiments using this gene and the heterologous U6 genes as probes indicate that the potato U6 gene family consists of more than 20 members. The potato U6 gene sequence shows high identity to previously characterized plant U6 snRNA gene sequences and possesses correctly positioned and spaced transcription control elements suggesting that it is an active gene.

Original languageEnglish
Pages (from-to)90-92
Number of pages3
JournalBBA - Gene Structure and Expression
Volume1129
Issue number1
DOIs
Publication statusPublished - 2 Dec 1991

Keywords

  • Potato
  • RNA processing
  • Small nuclear RNA
  • snRNA gene
  • U6 snRNA

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