Cloning and characterization of a U6 small nuclear RNA gene from potato

Yunqian Hu; John W.S. Brown; Robbie Waugh; Philip C. Turner

doi:10.1016/0167-4781(91)90217-A

Cloning and characterization of a U6 small nuclear RNA gene from potato

Yunqian Hu, John W.S. Brown, Robbie Waugh, Philip C. Turner (Lead / Corresponding author)

Plant Sciences

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

Abstract

Using a mixed U6 snRNA gene probe and a low stringency hybridization procedure we have isolated a U6 snRNA containing clone from a potato genomic library in λ EMBL 3. This clone cotains a single U6 snRNA gene which has been subcloned and sequenced. Southern blotting experiments using this gene and the heterologous U6 genes as probes indicate that the potato U6 gene family consists of more than 20 members. The potato U6 gene sequence shows high identity to previously characterized plant U6 snRNA gene sequences and possesses correctly positioned and spaced transcription control elements suggesting that it is an active gene.

Original language	English
Pages (from-to)	90-92
Number of pages	3
Journal	BBA - Gene Structure and Expression
Volume	1129
Issue number	1
DOIs	https://doi.org/10.1016/0167-4781(91)90217-A
Publication status	Published - 2 Dec 1991

Keywords

Potato
RNA processing
Small nuclear RNA
snRNA gene
U6 snRNA

ASJC Scopus subject areas

Structural Biology
Biophysics
Biochemistry
Genetics

Access to Document

10.1016/0167-4781(91)90217-A

Cite this

@article{bc12163ac7a04b079af5e3dfb0be886d,

title = "Cloning and characterization of a U6 small nuclear RNA gene from potato",

abstract = "Using a mixed U6 snRNA gene probe and a low stringency hybridization procedure we have isolated a U6 snRNA containing clone from a potato genomic library in λ EMBL 3. This clone cotains a single U6 snRNA gene which has been subcloned and sequenced. Southern blotting experiments using this gene and the heterologous U6 genes as probes indicate that the potato U6 gene family consists of more than 20 members. The potato U6 gene sequence shows high identity to previously characterized plant U6 snRNA gene sequences and possesses correctly positioned and spaced transcription control elements suggesting that it is an active gene.",

keywords = "Potato, RNA processing, Small nuclear RNA, snRNA gene, U6 snRNA",

author = "Yunqian Hu and Brown, {John W.S.} and Robbie Waugh and Turner, {Philip C.}",

year = "1991",

month = dec,

day = "2",

doi = "10.1016/0167-4781(91)90217-A",

language = "English",

volume = "1129",

pages = "90--92",

journal = "BBA - Gene Structure and Expression",

issn = "0167-4781",

publisher = "Elsevier",

number = "1",

}

TY - JOUR

T1 - Cloning and characterization of a U6 small nuclear RNA gene from potato

AU - Hu, Yunqian

AU - Brown, John W.S.

AU - Waugh, Robbie

AU - Turner, Philip C.

PY - 1991/12/2

Y1 - 1991/12/2

N2 - Using a mixed U6 snRNA gene probe and a low stringency hybridization procedure we have isolated a U6 snRNA containing clone from a potato genomic library in λ EMBL 3. This clone cotains a single U6 snRNA gene which has been subcloned and sequenced. Southern blotting experiments using this gene and the heterologous U6 genes as probes indicate that the potato U6 gene family consists of more than 20 members. The potato U6 gene sequence shows high identity to previously characterized plant U6 snRNA gene sequences and possesses correctly positioned and spaced transcription control elements suggesting that it is an active gene.

AB - Using a mixed U6 snRNA gene probe and a low stringency hybridization procedure we have isolated a U6 snRNA containing clone from a potato genomic library in λ EMBL 3. This clone cotains a single U6 snRNA gene which has been subcloned and sequenced. Southern blotting experiments using this gene and the heterologous U6 genes as probes indicate that the potato U6 gene family consists of more than 20 members. The potato U6 gene sequence shows high identity to previously characterized plant U6 snRNA gene sequences and possesses correctly positioned and spaced transcription control elements suggesting that it is an active gene.

KW - Potato

KW - RNA processing

KW - Small nuclear RNA

KW - snRNA gene

KW - U6 snRNA

UR - http://www.scopus.com/inward/record.url?scp=0025937648&partnerID=8YFLogxK

U2 - 10.1016/0167-4781(91)90217-A

DO - 10.1016/0167-4781(91)90217-A

M3 - Article

C2 - 1836741

AN - SCOPUS:0025937648

SN - 0167-4781

VL - 1129

SP - 90

EP - 92

JO - BBA - Gene Structure and Expression

JF - BBA - Gene Structure and Expression

IS - 1

ER -

Cloning and characterization of a U6 small nuclear RNA gene from potato

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this