Cloning and characterization of an orphan seven transmembrane receptor from Schistosoma mansoni

M. S. Pearson, D. P. McManus, D. J. Smyth, M. K. Jones, A. M. Sykes, A. Loukas

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

A partial cDNA sequence was obtained from the human blood fluke, Schistosoma mansoni using a signal sequence trap approach. The full-length cDNA was cloned and termed Sm-7TM. The corresponding open reading frame had 7 membrane spanning domains and shared identity with a small, novel group of seven transmembrane (7TM) receptors from vertebrates and invertebrates, including the human ee3 receptor - a heptahelical protein implicated in neuronal signalling. Phylogenetic analysis of this novel family showed that the Sm-7TM ORF formed a clade with exclusively invertebrate sequences. Based on topology modelling with ee3, Sm-7TM was predicted to possess an intracellular C-terminal tail, which was expressed as a soluble thioredoxin fusion protein (Sm-7TMC) in Escherichia coli and purified using metal ion-affinity chromatography. A polyclonal antiserum against this domain was used to detect Sm-7TM in detergent-soluble parasite extracts and to immunolocalize the receptor to the tegument of adult S. mansoni.

Original languageEnglish
Pages (from-to)2001-2008
Number of pages8
JournalParasitology
Volume134
Issue number14
DOIs
Publication statusPublished - 1 Dec 2007

Keywords

  • Schistosoma mansoni
  • Seven transmembrane receptor
  • Tegument

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