Cloning and fnctional expression of an apparent splice variant of the murine 5-HT3 receptor A subunit

Anthony G. Hope, David L. Downie, Lesley A. Sutherland, Jeremy J. Lambert, John A. Peters, Brian Burchell

Research output: Contribution to journalArticle

139 Citations (Scopus)

Abstract

The polymerase chain reaction has been employed to isolate a cDNA encoding a functional 5-HT3 receptor subunit from the murine neuroblastoma cell line N1E-115. Overall, the amino acid sequence predicted from this clone demonstrates a 98% homology with the 5-HT3 receptor A subunit cloned from NCB-20 hybridoma cells. A deletion of 6 amino acid residues located within the putative large intracellular loop, which may result from alternative splicing, represents the principal difference between the two clones. Upon epression in Xenopus oocytes, the homo-oligomeric receptor displayed pharmacological properties which define it as a functional 5-HT3 receptor.

Original languageEnglish
Pages (from-to)187-192
Number of pages6
JournalEuropean Journal of Pharmacology: Molecular Pharmacology
Volume245
Issue number2
DOIs
Publication statusPublished - 15 Apr 1993

Keywords

  • 5-HT receptor
  • Cloned 5-HT receptor subunit
  • N1E-115 neuroblastoma cell
  • Xenopus oocyte expression system

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