TY - JOUR
T1 - Cloning and fnctional expression of an apparent splice variant of the murine 5-HT3 receptor A subunit
AU - Hope, Anthony G.
AU - Downie, David L.
AU - Sutherland, Lesley A.
AU - Lambert, Jeremy J.
AU - Peters, John A.
AU - Burchell, Brian
PY - 1993/4/15
Y1 - 1993/4/15
N2 - The polymerase chain reaction has been employed to isolate a cDNA encoding a functional 5-HT3 receptor subunit from the murine neuroblastoma cell line N1E-115. Overall, the amino acid sequence predicted from this clone demonstrates a 98% homology with the 5-HT3 receptor A subunit cloned from NCB-20 hybridoma cells. A deletion of 6 amino acid residues located within the putative large intracellular loop, which may result from alternative splicing, represents the principal difference between the two clones. Upon epression in Xenopus oocytes, the homo-oligomeric receptor displayed pharmacological properties which define it as a functional 5-HT3 receptor.
AB - The polymerase chain reaction has been employed to isolate a cDNA encoding a functional 5-HT3 receptor subunit from the murine neuroblastoma cell line N1E-115. Overall, the amino acid sequence predicted from this clone demonstrates a 98% homology with the 5-HT3 receptor A subunit cloned from NCB-20 hybridoma cells. A deletion of 6 amino acid residues located within the putative large intracellular loop, which may result from alternative splicing, represents the principal difference between the two clones. Upon epression in Xenopus oocytes, the homo-oligomeric receptor displayed pharmacological properties which define it as a functional 5-HT3 receptor.
KW - 5-HT receptor
KW - Cloned 5-HT receptor subunit
KW - N1E-115 neuroblastoma cell
KW - Xenopus oocyte expression system
UR - http://www.scopus.com/inward/record.url?scp=0027511769&partnerID=8YFLogxK
U2 - 10.1016/0922-4106(93)90128-V
DO - 10.1016/0922-4106(93)90128-V
M3 - Article
C2 - 7683998
AN - SCOPUS:0027511769
SN - 0922-4106
VL - 245
SP - 187
EP - 192
JO - European Journal of Pharmacology: Molecular Pharmacology
JF - European Journal of Pharmacology: Molecular Pharmacology
IS - 2
ER -