Cloning and fnctional expression of an apparent splice variant of the murine 5-HT3 receptor A subunit

Anthony G. Hope; David L. Downie; Lesley A.  Sutherland; Jeremy J. Lambert; John A. Peters; Brian Burchell

doi:10.1016/0922-4106(93)90128-V

Cloning and fnctional expression of an apparent splice variant of the murine 5-HT3 receptor A subunit

Anthony G. Hope
, David L. Downie
, Lesley A. Sutherland
, Jeremy J. Lambert
, John A. Peters
, Brian Burchell

Research output: Contribution to journal › Article › peer-review

150 Citations (Scopus)

Abstract

The polymerase chain reaction has been employed to isolate a cDNA encoding a functional 5-HT₃ receptor subunit from the murine neuroblastoma cell line N1E-115. Overall, the amino acid sequence predicted from this clone demonstrates a 98% homology with the 5-HT₃ receptor A subunit cloned from NCB-20 hybridoma cells. A deletion of 6 amino acid residues located within the putative large intracellular loop, which may result from alternative splicing, represents the principal difference between the two clones. Upon epression in Xenopus oocytes, the homo-oligomeric receptor displayed pharmacological properties which define it as a functional 5-HT₃ receptor.

Original language	English
Pages (from-to)	187-192
Number of pages	6
Journal	European Journal of Pharmacology: Molecular Pharmacology
Volume	245
Issue number	2
DOIs	https://doi.org/10.1016/0922-4106(93)90128-V
Publication status	Published - 15 Apr 1993

Keywords

5-HT receptor
Cloned 5-HT receptor subunit
N1E-115 neuroblastoma cell
Xenopus oocyte expression system

ASJC Scopus subject areas

Pharmacology

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10.1016/0922-4106(93)90128-V

Cite this

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title = "Cloning and fnctional expression of an apparent splice variant of the murine 5-HT3 receptor A subunit",

abstract = "The polymerase chain reaction has been employed to isolate a cDNA encoding a functional 5-HT3 receptor subunit from the murine neuroblastoma cell line N1E-115. Overall, the amino acid sequence predicted from this clone demonstrates a 98\% homology with the 5-HT3 receptor A subunit cloned from NCB-20 hybridoma cells. A deletion of 6 amino acid residues located within the putative large intracellular loop, which may result from alternative splicing, represents the principal difference between the two clones. Upon epression in Xenopus oocytes, the homo-oligomeric receptor displayed pharmacological properties which define it as a functional 5-HT3 receptor.",

keywords = "5-HT receptor, Cloned 5-HT receptor subunit, N1E-115 neuroblastoma cell, Xenopus oocyte expression system",

author = "Hope, \{Anthony G.\} and Downie, \{David L.\} and Sutherland, \{Lesley A.\} and Lambert, \{Jeremy J.\} and Peters, \{John A.\} and Brian Burchell",

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doi = "10.1016/0922-4106(93)90128-V",

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T1 - Cloning and fnctional expression of an apparent splice variant of the murine 5-HT3 receptor A subunit

AU - Hope, Anthony G.

AU - Downie, David L.

AU - Sutherland, Lesley A.

AU - Lambert, Jeremy J.

AU - Peters, John A.

AU - Burchell, Brian

PY - 1993/4/15

Y1 - 1993/4/15

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AB - The polymerase chain reaction has been employed to isolate a cDNA encoding a functional 5-HT3 receptor subunit from the murine neuroblastoma cell line N1E-115. Overall, the amino acid sequence predicted from this clone demonstrates a 98% homology with the 5-HT3 receptor A subunit cloned from NCB-20 hybridoma cells. A deletion of 6 amino acid residues located within the putative large intracellular loop, which may result from alternative splicing, represents the principal difference between the two clones. Upon epression in Xenopus oocytes, the homo-oligomeric receptor displayed pharmacological properties which define it as a functional 5-HT3 receptor.

KW - 5-HT receptor

KW - Cloned 5-HT receptor subunit

KW - N1E-115 neuroblastoma cell

KW - Xenopus oocyte expression system

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JF - European Journal of Pharmacology: Molecular Pharmacology

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ER -

Cloning and fnctional expression of an apparent splice variant of the murine 5-HT3 receptor A subunit

Abstract

Keywords

ASJC Scopus subject areas

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