Cloning and functional expression of the cDNA.-encoding an inwardly-rectifying potassium channel expressed in pancreatic beta-cells and in the brain

C.T. Bond; C. Ammala; R. Ashfield; T. A. Blair; F. Gribble; R. N. Khan; K. Lee; P. Proks; I. C. M. Rowe; H. Sakura; M. J. Ashford; J. P. Adelman; F. M. Ashcroft

doi:10.1016/0014-5793(95)00497-W

Cloning and functional expression of the cDNA.-encoding an inwardly-rectifying potassium channel expressed in pancreatic beta-cells and in the brain

C.T. Bond
, C. Ammala
, R. Ashfield
, T. A. Blair
, F. Gribble
, R. N. Khan
, K. Lee
, P. Proks
, I. C. M. Rowe
, H. Sakura
, M. J. Ashford
, J. P. Adelman
, F. M. Ashcroft

Research output: Contribution to journal › Article › peer-review

46 Citations (Scopus)

Abstract

A cDNA clone encoding an inwardly-rectifying K-channel (BIR1) was isolated from insulinoma cells. The predicted amino acid sequence shares 72% identity with the cardiac ATP-sensitive K-channel rcK(ATP)(K-ATP-1; [6]). The mRNA is expressed in the brain and insulinoma cells. Heterologous expression in Xenopus oocytes produced currents which were K+-selective, time-independent and showed inward rectification. The currents were blocked by external barium and caesium, but insensitive to tolbutamide and diazoxide. In inside-out patches, channel activity was not blocked by 1 mM internal ATP. The sequence homology with K-ATP-1 suggests that BIR1 is a subunit of a brain and beta-cell K-ATP channel. However, pharmacological differences and the lack of ATP-sensitivity, suggest that if, this is the case, heterologous subunits must exert strong modulatory influences on the native channel.

Original language	English
Pages (from-to)	61-66
Number of pages	6
Journal	FEBS Letters
Volume	367
Issue number	1
DOIs	https://doi.org/10.1016/0014-5793(95)00497-W
Publication status	Published - 1995

Keywords

PANCREATIC BETA-CELL
GLUCOSE
INWARD RECTIFIER
ATP
BIR1
K-CHANNEL
ATP-SENSITIVE K-CHANNEL

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10.1016/0014-5793(95)00497-W

http://www.febsletters.org/article/0014-5793%2895%2900497-W/abstract

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Bond, C. T., Ammala, C., Ashfield, R., Blair, T. A., Gribble, F., Khan, R. N., Lee, K., Proks, P., Rowe, I. C. M., Sakura, H., Ashford, M. J., Adelman, J. P., & Ashcroft, F. M. (1995). Cloning and functional expression of the cDNA.-encoding an inwardly-rectifying potassium channel expressed in pancreatic beta-cells and in the brain. FEBS Letters, 367(1), 61-66. https://doi.org/10.1016/0014-5793(95)00497-W

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title = "Cloning and functional expression of the cDNA.-encoding an inwardly-rectifying potassium channel expressed in pancreatic beta-cells and in the brain",

abstract = "A cDNA clone encoding an inwardly-rectifying K-channel (BIR1) was isolated from insulinoma cells. The predicted amino acid sequence shares 72\% identity with the cardiac ATP-sensitive K-channel rcK(ATP)(K-ATP-1; [6]). The mRNA is expressed in the brain and insulinoma cells. Heterologous expression in Xenopus oocytes produced currents which were K+-selective, time-independent and showed inward rectification. The currents were blocked by external barium and caesium, but insensitive to tolbutamide and diazoxide. In inside-out patches, channel activity was not blocked by 1 mM internal ATP. The sequence homology with K-ATP-1 suggests that BIR1 is a subunit of a brain and beta-cell K-ATP channel. However, pharmacological differences and the lack of ATP-sensitivity, suggest that if, this is the case, heterologous subunits must exert strong modulatory influences on the native channel.",

keywords = "PANCREATIC BETA-CELL, GLUCOSE, INWARD RECTIFIER, ATP, BIR1, K-CHANNEL, ATP-SENSITIVE K-CHANNEL",

author = "C.T. Bond and C. Ammala and R. Ashfield and Blair, \{T. A.\} and F. Gribble and Khan, \{R. N.\} and K. Lee and P. Proks and Rowe, \{I. C. M.\} and H. Sakura and Ashford, \{M. J.\} and Adelman, \{J. P.\} and Ashcroft, \{F. M.\}",

year = "1995",

doi = "10.1016/0014-5793(95)00497-W",

language = "English",

volume = "367",

pages = "61--66",

journal = "FEBS Letters",

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publisher = "Wiley",

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Bond, CT, Ammala, C, Ashfield, R, Blair, TA, Gribble, F, Khan, RN, Lee, K, Proks, P, Rowe, ICM, Sakura, H, Ashford, MJ, Adelman, JP & Ashcroft, FM 1995, 'Cloning and functional expression of the cDNA.-encoding an inwardly-rectifying potassium channel expressed in pancreatic beta-cells and in the brain', FEBS Letters, vol. 367, no. 1, pp. 61-66. https://doi.org/10.1016/0014-5793(95)00497-W

TY - JOUR

T1 - Cloning and functional expression of the cDNA.-encoding an inwardly-rectifying potassium channel expressed in pancreatic beta-cells and in the brain

AU - Bond, C.T.

AU - Ammala, C.

AU - Ashfield, R.

AU - Blair, T. A.

AU - Gribble, F.

AU - Khan, R. N.

AU - Lee, K.

AU - Proks, P.

AU - Rowe, I. C. M.

AU - Sakura, H.

AU - Ashford, M. J.

AU - Adelman, J. P.

AU - Ashcroft, F. M.

PY - 1995

Y1 - 1995

N2 - A cDNA clone encoding an inwardly-rectifying K-channel (BIR1) was isolated from insulinoma cells. The predicted amino acid sequence shares 72% identity with the cardiac ATP-sensitive K-channel rcK(ATP)(K-ATP-1; [6]). The mRNA is expressed in the brain and insulinoma cells. Heterologous expression in Xenopus oocytes produced currents which were K+-selective, time-independent and showed inward rectification. The currents were blocked by external barium and caesium, but insensitive to tolbutamide and diazoxide. In inside-out patches, channel activity was not blocked by 1 mM internal ATP. The sequence homology with K-ATP-1 suggests that BIR1 is a subunit of a brain and beta-cell K-ATP channel. However, pharmacological differences and the lack of ATP-sensitivity, suggest that if, this is the case, heterologous subunits must exert strong modulatory influences on the native channel.

AB - A cDNA clone encoding an inwardly-rectifying K-channel (BIR1) was isolated from insulinoma cells. The predicted amino acid sequence shares 72% identity with the cardiac ATP-sensitive K-channel rcK(ATP)(K-ATP-1; [6]). The mRNA is expressed in the brain and insulinoma cells. Heterologous expression in Xenopus oocytes produced currents which were K+-selective, time-independent and showed inward rectification. The currents were blocked by external barium and caesium, but insensitive to tolbutamide and diazoxide. In inside-out patches, channel activity was not blocked by 1 mM internal ATP. The sequence homology with K-ATP-1 suggests that BIR1 is a subunit of a brain and beta-cell K-ATP channel. However, pharmacological differences and the lack of ATP-sensitivity, suggest that if, this is the case, heterologous subunits must exert strong modulatory influences on the native channel.

KW - PANCREATIC BETA-CELL

KW - GLUCOSE

KW - INWARD RECTIFIER

KW - ATP

KW - BIR1

KW - K-CHANNEL

KW - ATP-SENSITIVE K-CHANNEL

U2 - 10.1016/0014-5793(95)00497-W

DO - 10.1016/0014-5793(95)00497-W

M3 - Article

SN - 0014-5793

VL - 367

SP - 61

EP - 66

JO - FEBS Letters

JF - FEBS Letters

IS - 1

ER -

Cloning and functional expression of the cDNA.-encoding an inwardly-rectifying potassium channel expressed in pancreatic beta-cells and in the brain

Abstract

Keywords

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