Cloning and intracellular localization of the U2 small nuclear ribonucleoprotein auxiliary factor small subunit

M. Zhang; P. D. Zamore; M. Carmo-Fonseca; A. I. Lamond; M. R. Green

doi:10.1073/pnas.89.18.8769

Cloning and intracellular localization of the U2 small nuclear ribonucleoprotein auxiliary factor small subunit

M. Zhang, P. D. Zamore, M. Carmo-Fonseca, A. I. Lamond, M. R. Green

Research output: Contribution to journal › Article › peer-review

179 Citations (Scopus)

Abstract

U2 small nuclear ribonucleoprotein auxiliary factor (U2AF), an essential mammalian splicing factor, is composed of two subunits: a 65-kDa protein (U2AF⁶⁵), which binds the pre-mRNA polypyrimidine tract and is required for in vitro splicing, and an associated 35-kDa protein (U2AF³⁵). Here we report the isolation of a cDNA encoding U2AF³⁵. U2AF³⁵ contains sequence motifs found in several mammalian pre-mRNA splicing factors. We show directly that U2AF⁶⁵ and U2AF³⁵ interact with each other and delineate the regions of both proteins that mediate this interaction. Using anti-peptide antibodies against U2AF³⁵, we show that the protein has the intracellular distribution characteristic of U2AF⁶⁵. Both U2AF⁶⁵ and U2AF³⁵ are concentrated in a small number of nuclear foci corresponding to coiled bodies, subnuclear organelles first identified by light microscopy in 1903.

Original language	English
Pages (from-to)	8769-8773
Number of pages	5
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	89
Issue number	18
DOIs	https://doi.org/10.1073/pnas.89.18.8769
Publication status	Published - 15 Sept 1992

Keywords

arginine, serine rich
coiled body
RNA binding
spliceosome
splicing

ASJC Scopus subject areas

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title = "Cloning and intracellular localization of the U2 small nuclear ribonucleoprotein auxiliary factor small subunit",

abstract = "U2 small nuclear ribonucleoprotein auxiliary factor (U2AF), an essential mammalian splicing factor, is composed of two subunits: a 65-kDa protein (U2AF65), which binds the pre-mRNA polypyrimidine tract and is required for in vitro splicing, and an associated 35-kDa protein (U2AF35). Here we report the isolation of a cDNA encoding U2AF35. U2AF35 contains sequence motifs found in several mammalian pre-mRNA splicing factors. We show directly that U2AF65 and U2AF35 interact with each other and delineate the regions of both proteins that mediate this interaction. Using anti-peptide antibodies against U2AF35, we show that the protein has the intracellular distribution characteristic of U2AF65. Both U2AF65 and U2AF35 are concentrated in a small number of nuclear foci corresponding to coiled bodies, subnuclear organelles first identified by light microscopy in 1903.",

keywords = "arginine, serine rich, coiled body, RNA binding, spliceosome, splicing",

author = "M. Zhang and Zamore, {P. D.} and M. Carmo-Fonseca and Lamond, {A. I.} and Green, {M. R.}",

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T1 - Cloning and intracellular localization of the U2 small nuclear ribonucleoprotein auxiliary factor small subunit

AU - Zhang, M.

AU - Zamore, P. D.

AU - Carmo-Fonseca, M.

AU - Lamond, A. I.

AU - Green, M. R.

PY - 1992/9/15

Y1 - 1992/9/15

N2 - U2 small nuclear ribonucleoprotein auxiliary factor (U2AF), an essential mammalian splicing factor, is composed of two subunits: a 65-kDa protein (U2AF65), which binds the pre-mRNA polypyrimidine tract and is required for in vitro splicing, and an associated 35-kDa protein (U2AF35). Here we report the isolation of a cDNA encoding U2AF35. U2AF35 contains sequence motifs found in several mammalian pre-mRNA splicing factors. We show directly that U2AF65 and U2AF35 interact with each other and delineate the regions of both proteins that mediate this interaction. Using anti-peptide antibodies against U2AF35, we show that the protein has the intracellular distribution characteristic of U2AF65. Both U2AF65 and U2AF35 are concentrated in a small number of nuclear foci corresponding to coiled bodies, subnuclear organelles first identified by light microscopy in 1903.

AB - U2 small nuclear ribonucleoprotein auxiliary factor (U2AF), an essential mammalian splicing factor, is composed of two subunits: a 65-kDa protein (U2AF65), which binds the pre-mRNA polypyrimidine tract and is required for in vitro splicing, and an associated 35-kDa protein (U2AF35). Here we report the isolation of a cDNA encoding U2AF35. U2AF35 contains sequence motifs found in several mammalian pre-mRNA splicing factors. We show directly that U2AF65 and U2AF35 interact with each other and delineate the regions of both proteins that mediate this interaction. Using anti-peptide antibodies against U2AF35, we show that the protein has the intracellular distribution characteristic of U2AF65. Both U2AF65 and U2AF35 are concentrated in a small number of nuclear foci corresponding to coiled bodies, subnuclear organelles first identified by light microscopy in 1903.

KW - arginine, serine rich

KW - coiled body

KW - RNA binding

KW - spliceosome

KW - splicing

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DO - 10.1073/pnas.89.18.8769

M3 - Article

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AN - SCOPUS:0026649523

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JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

IS - 18

ER -

Cloning and intracellular localization of the U2 small nuclear ribonucleoprotein auxiliary factor small subunit

Abstract

Keywords

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