Cloning and intracellular localization of the U2 small nuclear ribonucleoprotein auxiliary factor small subunit

TY - JOUR

T1 - Cloning and intracellular localization of the U2 small nuclear ribonucleoprotein auxiliary factor small subunit

AU - Zhang, M.

AU - Zamore, P. D.

AU - Carmo-Fonseca, M.

AU - Lamond, A. I.

AU - Green, M. R.

PY - 1992/9/15

Y1 - 1992/9/15

N2 - U2 small nuclear ribonucleoprotein auxiliary factor (U2AF), an essential mammalian splicing factor, is composed of two subunits: a 65-kDa protein (U2AF65), which binds the pre-mRNA polypyrimidine tract and is required for in vitro splicing, and an associated 35-kDa protein (U2AF35). Here we report the isolation of a cDNA encoding U2AF35. U2AF35 contains sequence motifs found in several mammalian pre-mRNA splicing factors. We show directly that U2AF65 and U2AF35 interact with each other and delineate the regions of both proteins that mediate this interaction. Using anti-peptide antibodies against U2AF35, we show that the protein has the intracellular distribution characteristic of U2AF65. Both U2AF65 and U2AF35 are concentrated in a small number of nuclear foci corresponding to coiled bodies, subnuclear organelles first identified by light microscopy in 1903.

AB - U2 small nuclear ribonucleoprotein auxiliary factor (U2AF), an essential mammalian splicing factor, is composed of two subunits: a 65-kDa protein (U2AF65), which binds the pre-mRNA polypyrimidine tract and is required for in vitro splicing, and an associated 35-kDa protein (U2AF35). Here we report the isolation of a cDNA encoding U2AF35. U2AF35 contains sequence motifs found in several mammalian pre-mRNA splicing factors. We show directly that U2AF65 and U2AF35 interact with each other and delineate the regions of both proteins that mediate this interaction. Using anti-peptide antibodies against U2AF35, we show that the protein has the intracellular distribution characteristic of U2AF65. Both U2AF65 and U2AF35 are concentrated in a small number of nuclear foci corresponding to coiled bodies, subnuclear organelles first identified by light microscopy in 1903.

KW - arginine, serine rich

KW - coiled body

KW - RNA binding

KW - spliceosome

KW - splicing

UR - https://www.scopus.com/pages/publications/0026649523

U2 - 10.1073/pnas.89.18.8769

DO - 10.1073/pnas.89.18.8769

M3 - Article

C2 - 1388271

AN - SCOPUS:0026649523

SN - 0027-8424

VL - 89

SP - 8769

EP - 8773

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

IS - 18

ER -