Cloning and sequence analysis of laccase-enconing cDNA clones from tobacco

Marie Christine Kiefer-Meyer; Véronique Gomord; Ann O'Connell; Claire Halpin; Loïc Faye

doi:10.1016/0378-1119(96)00381-2

Cloning and sequence analysis of laccase-enconing cDNA clones from tobacco

Marie Christine Kiefer-Meyer, Véronique Gomord, Ann O'Connell, Claire Halpin, Loïc Faye (Lead / Corresponding author)

Research output: Contribution to journal › Article › peer-review

40 Citations (Scopus)

Abstract

Three laccase-encoding cDNAs were cloned from a tobacco stem cDNA library. One of them contains a full length sequence coding for a cationic laccase. The predicted polypeptide sequence shows 48% identity with sycamore laccase. Amino acid comparisons with other laccases and ascorbate oxidases have shown that this new plant laccase sequence also contains four potential copper binding regions which are highly conserved among the blue copper oxidases.

Original language	English
Pages (from-to)	205-207
Number of pages	3
Journal	Gene
Volume	178
Issue number	1-2
DOIs	https://doi.org/10.1016/0378-1119(96)00381-2
Publication status	Published - 31 Oct 1996

Keywords

cDNA sequence
Copper binding region
Gene cloning
Lignin biosynthesis
Multicopper oxidase
Nicotiana tabacum

ASJC Scopus subject areas

Genetics

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10.1016/0378-1119(96)00381-2

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title = "Cloning and sequence analysis of laccase-enconing cDNA clones from tobacco",

abstract = "Three laccase-encoding cDNAs were cloned from a tobacco stem cDNA library. One of them contains a full length sequence coding for a cationic laccase. The predicted polypeptide sequence shows 48% identity with sycamore laccase. Amino acid comparisons with other laccases and ascorbate oxidases have shown that this new plant laccase sequence also contains four potential copper binding regions which are highly conserved among the blue copper oxidases.",

keywords = "cDNA sequence, Copper binding region, Gene cloning, Lignin biosynthesis, Multicopper oxidase, Nicotiana tabacum",

author = "Kiefer-Meyer, {Marie Christine} and V{\'e}ronique Gomord and Ann O'Connell and Claire Halpin and Lo{\"i}c Faye",

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T1 - Cloning and sequence analysis of laccase-enconing cDNA clones from tobacco

AU - Kiefer-Meyer, Marie Christine

AU - Gomord, Véronique

AU - O'Connell, Ann

AU - Halpin, Claire

AU - Faye, Loïc

PY - 1996/10/31

Y1 - 1996/10/31

N2 - Three laccase-encoding cDNAs were cloned from a tobacco stem cDNA library. One of them contains a full length sequence coding for a cationic laccase. The predicted polypeptide sequence shows 48% identity with sycamore laccase. Amino acid comparisons with other laccases and ascorbate oxidases have shown that this new plant laccase sequence also contains four potential copper binding regions which are highly conserved among the blue copper oxidases.

AB - Three laccase-encoding cDNAs were cloned from a tobacco stem cDNA library. One of them contains a full length sequence coding for a cationic laccase. The predicted polypeptide sequence shows 48% identity with sycamore laccase. Amino acid comparisons with other laccases and ascorbate oxidases have shown that this new plant laccase sequence also contains four potential copper binding regions which are highly conserved among the blue copper oxidases.

KW - cDNA sequence

KW - Copper binding region

KW - Gene cloning

KW - Lignin biosynthesis

KW - Multicopper oxidase

KW - Nicotiana tabacum

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U2 - 10.1016/0378-1119(96)00381-2

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VL - 178

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EP - 207

JO - Gene

JF - Gene

IS - 1-2

ER -

Cloning and sequence analysis of laccase-enconing cDNA clones from tobacco

Abstract

Keywords

ASJC Scopus subject areas

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