Cloning, experession and chromosomal localization of a member of the human cytochrome P450IIC gene sub‐family

E. A. Shephard, I. R. Phillips, I. Santisteban, C. N.A. Palmer, S. Povey

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

We have isolatd and sequenced a cDNA clone (pB8) that codes for a novel member of the dcytochrome P450IIC sub‐family of man. Analysis, by Southern blot hybridization, of DNA isolated from a panel of nine independent human‐rodent somatic cell hybrids demonstrated that the corresponding gene (CYP2C) is located on human chromosome 10. Northern blot hybridization of RNA isolated from human livers revealed a 10‐fold inter‐indicidual variation in the expression of the gene.

Original languageEnglish
Pages (from-to)23-31
Number of pages9
JournalAnnals of Human Genetics
Volume53
Issue number1
DOIs
Publication statusPublished - Jan 1989

Fingerprint

Chromosomes, Human, Pair 10
Hybrid Cells
Human Chromosomes
Cytochromes
Southern Blotting
Northern Blotting
Organism Cloning
Complementary DNA
Clone Cells
RNA
Gene Expression
Liver
DNA
Genes
cytochrome P-450 CYP2C subfamily

Cite this

Shephard, E. A. ; Phillips, I. R. ; Santisteban, I. ; Palmer, C. N.A. ; Povey, S. / Cloning, experession and chromosomal localization of a member of the human cytochrome P450IIC gene sub‐family. In: Annals of Human Genetics. 1989 ; Vol. 53, No. 1. pp. 23-31.
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Cloning, experession and chromosomal localization of a member of the human cytochrome P450IIC gene sub‐family. / Shephard, E. A.; Phillips, I. R.; Santisteban, I.; Palmer, C. N.A.; Povey, S.

In: Annals of Human Genetics, Vol. 53, No. 1, 01.1989, p. 23-31.

Research output: Contribution to journalArticle

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AU - Povey, S.

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