Cloning, expression, and characterization of a cold-adapted lipase gene from an antarctic deep-sea psychrotrophic bacterium, Psychrobacter sp 7195

Keyphrases

• Deep Sea 100%
• Cloning Expression 100%
• Lipase Gene 100%
• Psychrobacter 100%
• Psychrotrophic Bacteria 100%
• Cold-active Lipase 100%
• Fe3+ 33%
• Amino Acids 33%
• Incubation 33%
• Enzyme Activity 33%
• Substrate Specificity 33%
• Molecular Mass 33%
• Upstream Open Reading Frame (uORF) 33%
• Recombinant 33%
• Ca2+ 33%
• Active Sites 33%
• Zn2+ 33%
• Fe2+ 33%
• High Activity 33%
• Mg2+ 33%
• Lipolysis 33%
• Detergent 33%
• Triton X-100 (TX-100) 33%
• Co2+ 33%
• Specificity Analysis 33%
• Nitrophenyl 33%
• Hg2+ 33%
• Myristate 33%
• Tween 80 33%
• Tween-40 33%
• Purified Enzymes 33%
• Column Chromatography 33%
• Acyl Groups 33%
• Consensus Motifs 33%
• Polyacrylamide Gel Electrophoresis 33%
• Deep-sea Sediment 33%
• Genomic DNA Library 33%
• Prydz Bay 33%
• Tributyrin 33%
• DEAE-Sepharose 33%
• Cold-adapted 33%
• Span 60 33%
• Span 40 33%
• Psychrotrophic 33%
• Lipolytic Enzymes 33%
• Chap 33%
• Trimyristin 33%
• Sephadex G-75 33%
• Optimal pH 33%
• Cold Shock Protein 33%

Biochemistry, Genetics and Molecular Biology

• Enzyme 100%
• Triacylglycerol Lipase 100%
• Pancreatic Lipase 100%
• Psychrobacter 100%
• Enzyme Activity 25%
• Polyacrylamide Gel Electrophoresis 25%
• Amino Acids 25%
• Molecular Weight 25%
• Myristic Acid 25%
• Enzyme Specificity 25%
• Active Site 25%
• Lipolysis 25%
• Open Reading Frame 25%
• DNA Library 25%
• Column Chromatography 25%
• DEAE-Sepharose 25%
• Sephadex 25%
• Genomics 25%
• Serine 25%