Cloning of cDNAs coding for rat hepatic microsomal UDP- glucuronyltransferases

Michael R. Jackson, Lee R. McCarthy, Robert B. Corser, Gordon C. Barr, Brian Burchell

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    Abstract

    Radioiodinated, affinity-purified, anti-UDP- glucuronyltransferase (UDPGT) antibodies have been used to isolate cDNAs coding for UDPGT(s) from a rat liver cDNA library cloned in the expression vector bacteriophage ?gt11. The sizes of 10 cloned cDNAs range from 0.3-3.1 kb. The identity of the cDNAs was confirmed by the hybrid-select translation and immunochemical analyses. Restriction mapping indicates that 2 classes of cDNA coding for different UDPGT mRNAs have been cloned.
    Original languageEnglish
    Pages (from-to)147-153
    Number of pages7
    JournalGene
    Volume34
    Issue number2-3
    DOIs
    Publication statusPublished - 1985

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    Jackson, M. R., McCarthy, L. R., Corser, R. B., Barr, G. C., & Burchell, B. (1985). Cloning of cDNAs coding for rat hepatic microsomal UDP- glucuronyltransferases. Gene, 34(2-3), 147-153. https://doi.org/10.1016/0378-1119(85)90122-2