Cloning of cDNAs coding for rat hepatic microsomal UDP- glucuronyltransferases

Michael R. Jackson; Lee R. McCarthy; Robert B. Corser; Gordon C. Barr; Brian Burchell

doi:10.1016/0378-1119(85)90122-2

Cloning of cDNAs coding for rat hepatic microsomal UDP- glucuronyltransferases

Michael R. Jackson, Lee R. McCarthy, Robert B. Corser, Gordon C. Barr, Brian Burchell

Research output: Contribution to journal › Article › peer-review

25 Citations (Scopus)

Abstract

Radioiodinated, affinity-purified, anti-UDP- glucuronyltransferase (UDPGT) antibodies have been used to isolate cDNAs coding for UDPGT(s) from a rat liver cDNA library cloned in the expression vector bacteriophage ?gt11. The sizes of 10 cloned cDNAs range from 0.3-3.1 kb. The identity of the cDNAs was confirmed by the hybrid-select translation and immunochemical analyses. Restriction mapping indicates that 2 classes of cDNA coding for different UDPGT mRNAs have been cloned.

Original language	English
Pages (from-to)	147-153
Number of pages	7
Journal	Gene
Volume	34
Issue number	2-3
DOIs	https://doi.org/10.1016/0378-1119(85)90122-2
Publication status	Published - 1985

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abstract = "Radioiodinated, affinity-purified, anti-UDP- glucuronyltransferase (UDPGT) antibodies have been used to isolate cDNAs coding for UDPGT(s) from a rat liver cDNA library cloned in the expression vector bacteriophage ?gt11. The sizes of 10 cloned cDNAs range from 0.3-3.1 kb. The identity of the cDNAs was confirmed by the hybrid-select translation and immunochemical analyses. Restriction mapping indicates that 2 classes of cDNA coding for different UDPGT mRNAs have been cloned.",

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AU - Corser, Robert B.

AU - Barr, Gordon C.

AU - Burchell, Brian

PY - 1985

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AB - Radioiodinated, affinity-purified, anti-UDP- glucuronyltransferase (UDPGT) antibodies have been used to isolate cDNAs coding for UDPGT(s) from a rat liver cDNA library cloned in the expression vector bacteriophage ?gt11. The sizes of 10 cloned cDNAs range from 0.3-3.1 kb. The identity of the cDNAs was confirmed by the hybrid-select translation and immunochemical analyses. Restriction mapping indicates that 2 classes of cDNA coding for different UDPGT mRNAs have been cloned.

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Cloning of cDNAs coding for rat hepatic microsomal UDP- glucuronyltransferases

Abstract

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