Cloning of cDNAs coding for rat hepatic microsomal UDP- glucuronyltransferases

TY - JOUR

T1 - Cloning of cDNAs coding for rat hepatic microsomal UDP- glucuronyltransferases

AU - Jackson, Michael R.

AU - McCarthy, Lee R.

AU - Corser, Robert B.

AU - Barr, Gordon C.

AU - Burchell, Brian

PY - 1985

Y1 - 1985

N2 - Radioiodinated, affinity-purified, anti-UDP- glucuronyltransferase (UDPGT) antibodies have been used to isolate cDNAs coding for UDPGT(s) from a rat liver cDNA library cloned in the expression vector bacteriophage ?gt11. The sizes of 10 cloned cDNAs range from 0.3-3.1 kb. The identity of the cDNAs was confirmed by the hybrid-select translation and immunochemical analyses. Restriction mapping indicates that 2 classes of cDNA coding for different UDPGT mRNAs have been cloned.

AB - Radioiodinated, affinity-purified, anti-UDP- glucuronyltransferase (UDPGT) antibodies have been used to isolate cDNAs coding for UDPGT(s) from a rat liver cDNA library cloned in the expression vector bacteriophage ?gt11. The sizes of 10 cloned cDNAs range from 0.3-3.1 kb. The identity of the cDNAs was confirmed by the hybrid-select translation and immunochemical analyses. Restriction mapping indicates that 2 classes of cDNA coding for different UDPGT mRNAs have been cloned.

UR - https://www.scopus.com/pages/publications/84886639236

U2 - 10.1016/0378-1119(85)90122-2

DO - 10.1016/0378-1119(85)90122-2

M3 - Article

AN - SCOPUS:84886639236

SN - 0378-1119

VL - 34

SP - 147

EP - 153

JO - Gene

JF - Gene

IS - 2-3

ER -