Cloning of murine RNA polymerase I-specific TAF factors: conserved interactions between the subunits of the species-specific transcription initiation factor TIF-IB/SL1

Jutta Heix, Joost C. B. M. Zomerdijk, Ali Ravanpay, Robert Tjian, Ingrid Grummt

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    Abstract

    Promoter selectivity for all three classes of eukaryotic RNA polymerases is brought about by multimeric protein complexes containing TATA box binding protein (TBP) and specific TBP-associated factors (TAFs), Unlike class II- and III-specific TBP-TAF complexes, the corresponding murine and human class I-specific transcription initiation factor TIF-IB/SL1 exhibits a pronounced selectivity for its homologous promoter, As a first step toward understanding the molecular basis of species-specific promoter recognition, we cloned the cDNAs encoding the three mouse pol I-specific TBP-associated factors (TAF(I)s) and compared the amino acid sequences of the murine TAF(I)s with their human counterparts, The four subunits from either species can form stable chimeric complexes that contain stoichiometric amounts of TBP and TAF(I)s, demonstrating that differences in the primary structure of human and mouse TAF(I)s do not dramatically alter the network of protein-protein contacts responsible for assembly of the multimeric complex, Thus, primate vs, rodent promoter selectivity mediated by the TBP-TAF(I) complex is likely to be the result of cumulative subtle differences between individual subunits that lead to species-specific properties of RNA polymerase I transcription.

    Original languageEnglish
    Pages (from-to)1733-1738
    Number of pages6
    JournalProceedings of the National Academy of Sciences of the United States of America
    Volume94
    Issue number5
    Publication statusPublished - 1997

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