Clusters, factories and domains: The complex structure of S-phase comes into focus

    Research output: Contribution to journalArticle

    31 Citations (Scopus)

    Abstract

    During S-phase of the cell cycle, chromosomal DNA is replicated according to a complex replication timing program, with megabase-sized domains replicating at different times. DNA fibre analysis reveals that clusters of adjacent replication origins fire near-synchronously. Analysis of replicating cells by light microscopy shows that DNA synthesis occurs in discrete foci or factories. The relationship between timing domains, origin clusters and replication foci is currently unclear. Recent work, using a hybrid Xenopus/hamster replication system, has shown that when CDK levels are manipulated during S-phase the activation of replication factories can be uncoupled from progression through the replication timing program. Here, we use data from this hybrid system to investigate potential relationships between timing domains, origin clusters and replication foci. We suggest that each timing domain typically comprises several replicon clusters, which are usually processed sequentially by replication factories. We discuss how replication might be regulated at different levels to create this complex organisation and the potential involvement of CDKs in this process.

    Original languageEnglish
    Pages (from-to)3218-3226
    Number of pages9
    JournalCell Cycle
    Volume9
    Issue number16
    DOIs
    Publication statusPublished - 15 Aug 2010

    Keywords

    • DNA replication
    • CDKs
    • S-phase
    • replication timing
    • replicon clusters
    • replication foci
    • replication factories
    • chromosome domains
    • replication timing domains
    • CELL-CYCLE REGULATION
    • DNA-REPLICATION
    • CHROMOSOMAL DOMAINS
    • REPLICON CLUSTERS
    • DORMANT ORIGINS
    • EXCESS MCM2-7
    • G1 PHASE
    • XENOPUS
    • ORGANIZATION
    • CHROMATIN

    Cite this