CNP/cGMP signaling regulates axon branching and growth by modulating microtubule polymerization

Caihong Xia, Minh Nguyen, Amy K. Garrison, Zhen Zhao, Zheng Wang, Calum Sutherland, Le Ma

    Research output: Contribution to journalArticle

    11 Citations (Scopus)

    Abstract

    The peptide hormone CNP has recently been found to positively regulate axon branching and growth via activation of cGMP signaling in embryonic dorsal root ganglion (DRG) neurons, but the cellular mechanisms mediating the regulation of these developmental processes have not been established. In this study, we provide evidence linking CNP/cGMP signaling to microtubule dynamics via the microtubule regulator CRMP2. First, phosphorylation of CRMP2 can be suppressed by cGMP activation in embryonic DRG neurons, and non-phosphorylated CRMP2 promotes axon branching and growth. In addition, real time analysis of growing microtubule ends indicates a similar correlation of CRMP2 phosphorylation and its activity in promoting microtubule polymerization rates and durations in both COS cells and DRG neuron growth cones. Moreover, direct activation of cGMP signaling leads to increased assembly of dynamic microtubules in DRG growth cones. Finally, low doses of a microtubule depolymerization drug nocodazole block CNP/cGMP-dependent axon branching and growth. Taken together, our results support a critical role of microtubule dynamics in mediating CNP/cGMP regulation of axonal development. © 2013 Wiley Periodicals, Inc.
    Original languageEnglish
    Pages (from-to)673-687
    Number of pages15
    JournalDevelopmental Neurobiology
    Volume73
    Issue number9
    Early online date24 Jun 2013
    DOIs
    Publication statusPublished - Sep 2013

    Fingerprint

    Microtubules
    Polymerization
    Axons
    Spinal Ganglia
    Growth
    Growth Cones
    Neurons
    Phosphorylation
    Nocodazole
    Peptide Hormones
    COS Cells
    Pharmaceutical Preparations

    Cite this

    Xia, Caihong ; Nguyen, Minh ; Garrison, Amy K. ; Zhao, Zhen ; Wang, Zheng ; Sutherland, Calum ; Ma, Le. / CNP/cGMP signaling regulates axon branching and growth by modulating microtubule polymerization. In: Developmental Neurobiology. 2013 ; Vol. 73, No. 9. pp. 673-687.
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    abstract = "The peptide hormone CNP has recently been found to positively regulate axon branching and growth via activation of cGMP signaling in embryonic dorsal root ganglion (DRG) neurons, but the cellular mechanisms mediating the regulation of these developmental processes have not been established. In this study, we provide evidence linking CNP/cGMP signaling to microtubule dynamics via the microtubule regulator CRMP2. First, phosphorylation of CRMP2 can be suppressed by cGMP activation in embryonic DRG neurons, and non-phosphorylated CRMP2 promotes axon branching and growth. In addition, real time analysis of growing microtubule ends indicates a similar correlation of CRMP2 phosphorylation and its activity in promoting microtubule polymerization rates and durations in both COS cells and DRG neuron growth cones. Moreover, direct activation of cGMP signaling leads to increased assembly of dynamic microtubules in DRG growth cones. Finally, low doses of a microtubule depolymerization drug nocodazole block CNP/cGMP-dependent axon branching and growth. Taken together, our results support a critical role of microtubule dynamics in mediating CNP/cGMP regulation of axonal development. {\circledC} 2013 Wiley Periodicals, Inc.",
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    CNP/cGMP signaling regulates axon branching and growth by modulating microtubule polymerization. / Xia, Caihong; Nguyen, Minh; Garrison, Amy K.; Zhao, Zhen; Wang, Zheng; Sutherland, Calum; Ma, Le.

    In: Developmental Neurobiology, Vol. 73, No. 9, 09.2013, p. 673-687.

    Research output: Contribution to journalArticle

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