Co-ordinated gene expression during phases of dormancy release in raspberry (Rubus idaeus L.) buds

Luca Mazzitelli, Robert D. Hancock, Sophie Haupt, Paul G. Walker, Simon D. A. Pont, Jim McNicol, Linda Cardle, Jenny Morris, Roberto Viola, Rex Brennan, Peter E. Hedley, Mark A. Taylor

    Research output: Contribution to journalArticlepeer-review

    162 Citations (Scopus)

    Abstract

    Bud break in raspberry (Rubus idaeus L.) is often poor and uneven, with many of the subapical buds remaining in a dormant state. In order to determine the dormancy status of raspberry buds, an empirical measure of bud burst in a growth-permissive environment following exposure to chilling (4 degrees C cold storage) was developed. For cv. Glen Ample, percentage bud burst in intact canes and isolated nodes was recorded after 14 d. Isolated nodes (a measure of endodormancy) achieved 100% bud burst after approximately 1500 h chilling whereas buds on intact plants (combined endo- and paradormancy) required an additional 1000 h chilling. A microarray approach was used to follow changes in gene expression that occurred during dormancy transition. The probes for the microarrays were obtained from endodormant and paradormant raspberry bud cDNA libraries. The expression profiles of 5300 clones from these libraries were subjected to principal component analysis to determine the most significant expression patterns. Sequence analysis of these clones, in many cases, enabled their functional categorization and the development of hypotheses concerning the mechanisms of bud dormancy release. Thus a set of novel candidates for key dormancy-related genes from raspberry buds have been identified. Bud dormancy is fundamental to the study of plant developmental processes and, in addition, its regulation is of significant economic importance to fruit and horticultural industries.
    Original languageEnglish
    Pages (from-to)1035-1045
    Number of pages11
    JournalJournal of Experimental Botany
    Volume58
    Issue number5
    DOIs
    Publication statusPublished - Mar 2007

    Keywords

    • DNA, Plant
    • Expressed Sequence Tags
    • Flowers
    • Gene Expression Profiling
    • Gene Expression Regulation, Plant
    • Gene Library
    • Meristem
    • Oligonucleotide Array Sequence Analysis
    • Rosaceae
    • Temperature
    • Time Factors

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