Cocaine effects on mouse incentive-learning and human addiction are linked to alpha 2 subunit-containing GABA(A) receptors

Claire I. Dixon, Hannah V. Morris, Gerome Breen, Sylvane Desrivieres, Sarah Jugurnauth, Rebecca C. Steiner, Homero Vallada, Camila Guindalini, Ronaldo Laranjeira, Guilherme Messas, Thomas W. Rosahl, John R. Atack, Dianne R. Peden, Delia Belelli, Jeremy J. Lambert, Sarah L. King, Gunter Schumann, David N. Stephens (Lead / Corresponding author)

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    67 Citations (Scopus)


    Because GABA(A) receptors containing alpha 2 subunits are highly represented in areas of the brain, such as nucleus accumbens (NAcc), frontal cortex, and amygdala, regions intimately involved in signaling motivation and reward, we hypothesized that manipulations of this receptor subtype would influence processing of rewards. Voltage-clamp recordings from NAcc medium spiny neurons of mice with alpha 2 gene deletion showed reduced synaptic GABA(A) receptor-mediated responses. Behaviorally, the deletion abolished cocaine's ability to potentiate behaviors conditioned to rewards (conditioned reinforcement), and to support behavioral sensitization. In mice with a point mutation in the benzodiazepine binding pocket of alpha 2-GABA(A) receptors (alpha 2H101R), GABAergic neurotransmission in medium spiny neurons was identical to that of WT (i.e., the mutation was silent), but importantly, receptor function was now facilitated by the atypical benzodiazepine Ro 15-4513 (ethyl 8-amido-5,6-dihydro-5-methyl-6-oxo-4H-imidazo [1,5-a] [1,4] benzodiazepine-3-carboxylate). In alpha 2H101R, but not WT mice, Ro 15-4513 administered directly into the NAcc-stimulated locomotor activity, and when given systemically and repeatedly, induced behavioral sensitization. These data indicate that activation of alpha 2-GABA(A) receptors (most likely in NAcc) is both necessary and sufficient for behavioral sensitization. Consistent with a role of these receptors in addiction, we found specific markers and haplotypes of the GABRA2 gene to be associated with human cocaine addiction.

    Original languageEnglish
    Pages (from-to)2289-2294
    Number of pages6
    JournalProceedings of the National Academy of Sciences of the United States of America
    Issue number5
    Publication statusPublished - 2 Feb 2010


    • GABRA2
    • behavioral sensitization
    • nucleus accumbens
    • mutant mouse
    • human genetics
    • REWARD
    • BRAIN


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