Codon usage bias controls mRNA and protein abundance in trypanosomatids

Laura Jeacock, Joana Correia Faria, David Horn (Lead / Corresponding author)

Research output: Contribution to journalArticle

28 Citations (Scopus)
151 Downloads (Pure)

Abstract

Protein abundance differs from a few to millions of copies per cell. Trypanosoma brucei presents an excellent model for studies on codon bias and differential gene expression because transcription is broadly unregulated and uniform across the genome. T.brucei is also a major human and animal protozoal pathogen. Here, an experimental assessment, using synthetic reporter genes, revealed that GC3 codons have a major positive impact on both mRNA and protein abundance. Our estimates of relative expression, based on coding sequences alone (codon usage and sequence length), are within 2-fold of the observed values for the majority of measured cellular mRNAs (n>7000) and proteins (n>2000). Our estimates also correspond with expression measures from published transcriptome and proteome datasets from other trypanosomatids. We conclude that codon usage is a key factor affecting global relative mRNA and protein expression in trypanosomatids and that relative abundance can be effectively estimated using only protein coding sequences.
Original languageEnglish
Article numbere32496
Number of pages20
JournaleLife
Volume7
Early online date15 Mar 2018
DOIs
Publication statusPublished - 15 Mar 2018

Keywords

  • Leishmania
  • Post-transcription
  • Synonymous
  • Translation
  • Trypanosoma brucei
  • Trypanosoma vivas
  • Trypanosomes

Fingerprint Dive into the research topics of 'Codon usage bias controls mRNA and protein abundance in trypanosomatids'. Together they form a unique fingerprint.

  • Profiles

    Cite this