Collagen gel assay for angiogenesis: induction of endothelial cell sprouting

A M Schor, I Ellis, S L Schor

    Research output: Chapter in Book/Report/Conference proceedingChapter

    Abstract

    The inner lining of blood vessels, the endothelium, consists of a monolayer of endothelial cells (ECs), that present a free luminal surface and attach on their abluminal side to the underlying basement membrane (apart from a minimal amount of cell-cell overlap). A great deal of heterogeneity exists in the morphology of the endothelium and in the phenotype displayed by individual ECs. In spite of this, all ECs may be defined by two general criteria: anatomical location (i.e., luminal wall of blood vessels) and functionality (e.g., provision of a nonthrombogenic surface). In a mature resting vessel, the functionality and integrity of the endothelium is maintained under steady state conditions by the biosynthetic activity of the ECs, in conjunction with low levels of cell proliferation and motility. Significant changes in the motility of the endothelial cells, often accompanied by cell proliferation, occur during angiogenesis and in response to vessel injury.

    Original languageEnglish
    Title of host publicationAngiogenesis Protocols
    EditorsJ. C. Murray
    PublisherHumana Press
    Pages145-62
    Number of pages18
    ISBN (Electronic)978-1-59259-143-5
    ISBN (Print)978-0-89603-698-7
    DOIs
    Publication statusPublished - 2001

    Publication series

    NameMathods in Molecular Medicine
    PublisherHumana Press
    Volume46
    ISSN (Print)1543-1894

    Keywords

    • Maintenance Medium
    • Dialysis Tubing
    • Sodium Bicarbonate Solution
    • Laminar Flow Cabinet
    • Tissue Culture Incubator

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