Collection and characterisation of bacterial membrane proteins

Massoud Saidijam; Georgios Psakis; Joanne L Clough; Johan Meuller; Shun'ichi Suzuki; Christopher J Hoyle; Sarah L Palmer; Scott M Morrison; Martin K Pos; Richard C Essenberg; Martin C J Maiden; Atif Abu-bakr; Simon G Baumberg; Alex A Neyfakh; Jeffrey K Griffith; Michael J Stark; Alison Ward; John O'Reilly; Nicholas G Rutherford; Mary K Phillips-Jones; Peter J F Henderson; Gunnar von Heijne; Jan Rydstrom; Pater Brzezinski

doi:10.1016/S0014-5793(03)01148-7

Collection and characterisation of bacterial membrane proteins

Massoud Saidijam, Georgios Psakis, Joanne L Clough, Johan Meuller, Shun'ichi Suzuki, Christopher J Hoyle, Sarah L Palmer, Scott M Morrison, Martin K Pos, Richard C Essenberg, Martin C J Maiden, Atif Abu-bakr, Simon G Baumberg, Alex A Neyfakh, Jeffrey K Griffith, Michael J Stark, Alison Ward, John O'Reilly, Nicholas G Rutherford, Mary K Phillips-JonesPeter J F Henderson, Gunnar von Heijne (Editor), Jan Rydstrom (Editor), Pater Brzezinski (Editor)

Research output: Contribution to journal › Article › peer-review

32 Citations (Scopus)

Abstract

A general strategy for the amplified expression in Escherichia coli of membrane transport and receptor proteins from other bacteria is described. As an illustration we report the cloning of the putative a-ketoglutarate membrane transport gene from the genome of Helicobacter pylori, overexpression of the protein tagged with RGS(His)₆ at the C-terminus, and its purification in mg quantities. The retention of structural and functional integrity was verified by circular dichroism spectroscopy and reconstitution of transport activity. This strategy for overexpression and purification is extended to additional membrane proteins from H. pylori and from other bacteria.

Original language	English
Pages (from-to)	170-5
Number of pages	6
Journal	FEBS Letters
Volume	555
Issue number	1
DOIs	https://doi.org/10.1016/S0014-5793(03)01148-7
Publication status	Published - 27 Nov 2003

Keywords

membrane transport protein
alpha-ketoglutarate
two-component system
Pathogen
heliobacter pylori

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10.1016/S0014-5793(03)01148-7

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Saidijam, M., Psakis, G., Clough, J. L., Meuller, J., Suzuki, S., Hoyle, C. J., Palmer, S. L., Morrison, S. M., Pos, M. K., Essenberg, R. C., Maiden, M. C. J., Abu-bakr, A., Baumberg, S. G., Neyfakh, A. A., Griffith, J. K., Stark, M. J., Ward, A., O'Reilly, J., Rutherford, N. G., ... Brzezinski, P. (Ed.) (2003). Collection and characterisation of bacterial membrane proteins. FEBS Letters, 555(1), 170-5. https://doi.org/10.1016/S0014-5793(03)01148-7

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abstract = "A general strategy for the amplified expression in Escherichia coli of membrane transport and receptor proteins from other bacteria is described. As an illustration we report the cloning of the putative a-ketoglutarate membrane transport gene from the genome of Helicobacter pylori, overexpression of the protein tagged with RGS(His)6 at the C-terminus, and its purification in mg quantities. The retention of structural and functional integrity was verified by circular dichroism spectroscopy and reconstitution of transport activity. This strategy for overexpression and purification is extended to additional membrane proteins from H. pylori and from other bacteria.",

keywords = "membrane transport protein, alpha-ketoglutarate, two-component system, Pathogen, heliobacter pylori",

author = "Massoud Saidijam and Georgios Psakis and Clough, {Joanne L} and Johan Meuller and Shun'ichi Suzuki and Hoyle, {Christopher J} and Palmer, {Sarah L} and Morrison, {Scott M} and Pos, {Martin K} and Essenberg, {Richard C} and Maiden, {Martin C J} and Atif Abu-bakr and Baumberg, {Simon G} and Neyfakh, {Alex A} and Griffith, {Jeffrey K} and Stark, {Michael J} and Alison Ward and John O'Reilly and Rutherford, {Nicholas G} and Phillips-Jones, {Mary K} and Henderson, {Peter J F} and {von Heijne}, Gunnar and Jan Rydstrom and Pater Brzezinski",

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doi = "10.1016/S0014-5793(03)01148-7",

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Saidijam, M, Psakis, G, Clough, JL, Meuller, J, Suzuki, S, Hoyle, CJ, Palmer, SL, Morrison, SM, Pos, MK, Essenberg, RC, Maiden, MCJ, Abu-bakr, A, Baumberg, SG, Neyfakh, AA, Griffith, JK, Stark, MJ, Ward, A, O'Reilly, J, Rutherford, NG, Phillips-Jones, MK, Henderson, PJF, von Heijne, G (ed.), Rydstrom, J (ed.) & Brzezinski, P (ed.) 2003, 'Collection and characterisation of bacterial membrane proteins', FEBS Letters, vol. 555, no. 1, pp. 170-5. https://doi.org/10.1016/S0014-5793(03)01148-7

TY - JOUR

T1 - Collection and characterisation of bacterial membrane proteins

AU - Saidijam, Massoud

AU - Psakis, Georgios

AU - Clough, Joanne L

AU - Meuller, Johan

AU - Suzuki, Shun'ichi

AU - Hoyle, Christopher J

AU - Palmer, Sarah L

AU - Morrison, Scott M

AU - Pos, Martin K

AU - Essenberg, Richard C

AU - Maiden, Martin C J

AU - Abu-bakr, Atif

AU - Baumberg, Simon G

AU - Neyfakh, Alex A

AU - Griffith, Jeffrey K

AU - Stark, Michael J

AU - Ward, Alison

AU - O'Reilly, John

AU - Rutherford, Nicholas G

AU - Phillips-Jones, Mary K

AU - Henderson, Peter J F

A2 - von Heijne, Gunnar

A2 - Rydstrom, Jan

A2 - Brzezinski, Pater

PY - 2003/11/27

Y1 - 2003/11/27

N2 - A general strategy for the amplified expression in Escherichia coli of membrane transport and receptor proteins from other bacteria is described. As an illustration we report the cloning of the putative a-ketoglutarate membrane transport gene from the genome of Helicobacter pylori, overexpression of the protein tagged with RGS(His)6 at the C-terminus, and its purification in mg quantities. The retention of structural and functional integrity was verified by circular dichroism spectroscopy and reconstitution of transport activity. This strategy for overexpression and purification is extended to additional membrane proteins from H. pylori and from other bacteria.

AB - A general strategy for the amplified expression in Escherichia coli of membrane transport and receptor proteins from other bacteria is described. As an illustration we report the cloning of the putative a-ketoglutarate membrane transport gene from the genome of Helicobacter pylori, overexpression of the protein tagged with RGS(His)6 at the C-terminus, and its purification in mg quantities. The retention of structural and functional integrity was verified by circular dichroism spectroscopy and reconstitution of transport activity. This strategy for overexpression and purification is extended to additional membrane proteins from H. pylori and from other bacteria.

KW - membrane transport protein

KW - alpha-ketoglutarate

KW - two-component system

KW - Pathogen

KW - heliobacter pylori

U2 - 10.1016/S0014-5793(03)01148-7

DO - 10.1016/S0014-5793(03)01148-7

M3 - Article

C2 - 14630338

SN - 0014-5793

VL - 555

SP - 170

EP - 175

JO - FEBS Letters

JF - FEBS Letters

IS - 1

ER -

Collection and characterisation of bacterial membrane proteins

Abstract

Keywords

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