Colony PCR

Megan Bergkessel, Christine Guthrie

Research output: Chapter in Book/Report/Conference proceedingChapter

26 Citations (Scopus)

Abstract

Colony PCR is a method for rapidly screening colonies of yeast or bacteria that have grown up on selective media following a transformation step, to verify that the desired genetic construct is present, or to amplify a portion of the construct.

Original languageEnglish
Title of host publicationLaboratory Methods in Enzymology
Subtitle of host publicationDNA
EditorsJon Lorsch
PublisherAcademic Press Inc.
Chapter25
Pages299-309
Number of pages11
Volume529
ISBN (Print)9780124186873
DOIs
Publication statusPublished - 2013

Publication series

NameMethods in Enzymology
Volume529
ISSN (Print)0076-6879
ISSN (Electronic)1557-7988

Keywords

  • Colony PCR
  • DNA extraction
  • DNA-intercalating dye
  • Primer synthesis
  • Product visualization
  • Thermostable polymerase

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  • Cite this

    Bergkessel, M., & Guthrie, C. (2013). Colony PCR. In J. Lorsch (Ed.), Laboratory Methods in Enzymology: DNA (Vol. 529, pp. 299-309). (Methods in Enzymology; Vol. 529). Academic Press Inc.. https://doi.org/10.1016/B978-0-12-418687-3.00025-2