Comparative analysis of human papillomavirus detection by dot blot hybridisation and non-isotopic in situ hybridisation

G. Troncone, S. M. Anderson, C. S. Herrington, M. L. de Angelis, H. Noell, J. A. Chimera, J. O'D. McGee

    Research output: Contribution to journalArticle

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    Abstract

    Aim: To determine the relative diagnostic performance of non-isotopic in situ hybridisation (NISH) and a dot-blot assay for detecting human papillomavirus (HPV) on exfoliated cervical cells; and to correlate the results with cytopathological assessment.

    Methods: Cervical smears and cytological samples were obtained from 122 patients during the same clinical examination and the presence of HPV sequences determined by NISH and dot-blot analysis, respectively.

    Results: Dot-blot analysis gave an autoradiographic signal in 15 of 121 (12.4%) cases, while NISH detected viral genomes in 38 of 114 (33.3%) cases. Even in the presence of koilocytosis, where vegetative replication of the virus occurs, NISH was positive in over twice as many cases as dot-blot analysis (NISH 90%, dot-blot 40%), while in smears within normal cytological limits, where the viral copy number is likely to be considerably lower, the differences were more striking (NISH 31%, dot-blot 5%).

    Conclusions: These data show that NISH on cytological smears is more sensitive than a standardised dot-blot hybridisation assay for detecting HPV infection in cytological material and is therefore a more appropriate screening tool.

    Original languageEnglish
    Pages (from-to)866-870
    Number of pages5
    JournalJournal of Clinical Pathology
    Volume45
    Issue number10
    DOIs
    Publication statusPublished - Oct 1992

    Cite this

    Troncone, G., Anderson, S. M., Herrington, C. S., de Angelis, M. L., Noell, H., Chimera, J. A., & McGee, J. OD. (1992). Comparative analysis of human papillomavirus detection by dot blot hybridisation and non-isotopic in situ hybridisation. Journal of Clinical Pathology, 45(10), 866-870. https://doi.org/10.1136/jcp.45.10.866
    Troncone, G. ; Anderson, S. M. ; Herrington, C. S. ; de Angelis, M. L. ; Noell, H. ; Chimera, J. A. ; McGee, J. O'D. / Comparative analysis of human papillomavirus detection by dot blot hybridisation and non-isotopic in situ hybridisation. In: Journal of Clinical Pathology. 1992 ; Vol. 45, No. 10. pp. 866-870.
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    title = "Comparative analysis of human papillomavirus detection by dot blot hybridisation and non-isotopic in situ hybridisation",
    abstract = "Aim: To determine the relative diagnostic performance of non-isotopic in situ hybridisation (NISH) and a dot-blot assay for detecting human papillomavirus (HPV) on exfoliated cervical cells; and to correlate the results with cytopathological assessment.Methods: Cervical smears and cytological samples were obtained from 122 patients during the same clinical examination and the presence of HPV sequences determined by NISH and dot-blot analysis, respectively.Results: Dot-blot analysis gave an autoradiographic signal in 15 of 121 (12.4{\%}) cases, while NISH detected viral genomes in 38 of 114 (33.3{\%}) cases. Even in the presence of koilocytosis, where vegetative replication of the virus occurs, NISH was positive in over twice as many cases as dot-blot analysis (NISH 90{\%}, dot-blot 40{\%}), while in smears within normal cytological limits, where the viral copy number is likely to be considerably lower, the differences were more striking (NISH 31{\%}, dot-blot 5{\%}).Conclusions: These data show that NISH on cytological smears is more sensitive than a standardised dot-blot hybridisation assay for detecting HPV infection in cytological material and is therefore a more appropriate screening tool.",
    author = "G. Troncone and Anderson, {S. M.} and Herrington, {C. S.} and {de Angelis}, {M. L.} and H. Noell and Chimera, {J. A.} and McGee, {J. O'D.}",
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    Troncone, G, Anderson, SM, Herrington, CS, de Angelis, ML, Noell, H, Chimera, JA & McGee, JOD 1992, 'Comparative analysis of human papillomavirus detection by dot blot hybridisation and non-isotopic in situ hybridisation', Journal of Clinical Pathology, vol. 45, no. 10, pp. 866-870. https://doi.org/10.1136/jcp.45.10.866

    Comparative analysis of human papillomavirus detection by dot blot hybridisation and non-isotopic in situ hybridisation. / Troncone, G.; Anderson, S. M.; Herrington, C. S.; de Angelis, M. L.; Noell, H.; Chimera, J. A.; McGee, J. O'D.

    In: Journal of Clinical Pathology, Vol. 45, No. 10, 10.1992, p. 866-870.

    Research output: Contribution to journalArticle

    TY - JOUR

    T1 - Comparative analysis of human papillomavirus detection by dot blot hybridisation and non-isotopic in situ hybridisation

    AU - Troncone, G.

    AU - Anderson, S. M.

    AU - Herrington, C. S.

    AU - de Angelis, M. L.

    AU - Noell, H.

    AU - Chimera, J. A.

    AU - McGee, J. O'D.

    PY - 1992/10

    Y1 - 1992/10

    N2 - Aim: To determine the relative diagnostic performance of non-isotopic in situ hybridisation (NISH) and a dot-blot assay for detecting human papillomavirus (HPV) on exfoliated cervical cells; and to correlate the results with cytopathological assessment.Methods: Cervical smears and cytological samples were obtained from 122 patients during the same clinical examination and the presence of HPV sequences determined by NISH and dot-blot analysis, respectively.Results: Dot-blot analysis gave an autoradiographic signal in 15 of 121 (12.4%) cases, while NISH detected viral genomes in 38 of 114 (33.3%) cases. Even in the presence of koilocytosis, where vegetative replication of the virus occurs, NISH was positive in over twice as many cases as dot-blot analysis (NISH 90%, dot-blot 40%), while in smears within normal cytological limits, where the viral copy number is likely to be considerably lower, the differences were more striking (NISH 31%, dot-blot 5%).Conclusions: These data show that NISH on cytological smears is more sensitive than a standardised dot-blot hybridisation assay for detecting HPV infection in cytological material and is therefore a more appropriate screening tool.

    AB - Aim: To determine the relative diagnostic performance of non-isotopic in situ hybridisation (NISH) and a dot-blot assay for detecting human papillomavirus (HPV) on exfoliated cervical cells; and to correlate the results with cytopathological assessment.Methods: Cervical smears and cytological samples were obtained from 122 patients during the same clinical examination and the presence of HPV sequences determined by NISH and dot-blot analysis, respectively.Results: Dot-blot analysis gave an autoradiographic signal in 15 of 121 (12.4%) cases, while NISH detected viral genomes in 38 of 114 (33.3%) cases. Even in the presence of koilocytosis, where vegetative replication of the virus occurs, NISH was positive in over twice as many cases as dot-blot analysis (NISH 90%, dot-blot 40%), while in smears within normal cytological limits, where the viral copy number is likely to be considerably lower, the differences were more striking (NISH 31%, dot-blot 5%).Conclusions: These data show that NISH on cytological smears is more sensitive than a standardised dot-blot hybridisation assay for detecting HPV infection in cytological material and is therefore a more appropriate screening tool.

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    SN - 0021-9746

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