Comparative Transcriptomic Analysis of the Burkholderia cepacia Tyrosine Kinase bceF Mutant Reveals a Role in Tolerance to Stress, Biofilm Formation, and Virulence

Ana S. Ferreira, Ines N. Silva, Vitor H. Oliveira, Joerg D. Becker, Michael Givskov, Robert P. Ryan, Fabio Fernandes, Leonilde M. Moreira

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    Abstract

    The bacterial tyrosine-kinase (BY-kinase) family comprises the major group of bacterial enzymes endowed with tyrosine kinase activity. We previously showed that the BceF protein from Burkholderia cepacia IST408 belongs to this BY-kinase family and is involved in the biosynthesis of the exopolysaccharide cepacian. However, little is known about the extent of regulation of this protein kinase activity. In order to examine this regulation, we performed a comparative transcriptome profile between the bceF mutant and wild-type B. cepacia IST408. The analyses led to identification of 630 genes whose expression was significantly changed. Genes with decreased expression in the bceF mutant were related to stress response, motility, cell adhesion, and carbon and energy metabolism. Genes with increased expression were related to intracellular signaling and lipid metabolism. Mutation of bceF led to reduced survival under heat shock and UV light exposure, reduced swimming motility, and alteration in biofilm architecture when grown in vitro. Consistent with some of these phenotypes, the bceF mutant demonstrated elevated levels of cyclic-di-GMP. Furthermore, BceF contributed to the virulence of B. cepacia for larvae of the Greater wax moth, Galleria mellonella. Taken together, BceF appears to play a considerable role in many cellular processes, including biofilm formation and virulence. As homologues of BceF occur in a number of pathogenic and plant-associated Burkholderia strains, the modulation of bacterial behavior through tyrosine kinase activity is most likely a widely occurring phenomenon.

    Original languageEnglish
    Pages (from-to)3009-3020
    Number of pages12
    JournalApplied and Environmental Microbiology
    Volume79
    Issue number9
    DOIs
    Publication statusPublished - 2013

    Keywords

    • CYSTIC-FIBROSIS
    • ESCHERICHIA-COLI
    • MODEL
    • COMPLEX
    • BACILLUS-SUBTILIS
    • HEAT-SHOCK RESPONSE
    • OLIGONUCLEOTIDE ARRAYS
    • EXOPOLYSACCHARIDE BIOSYNTHESIS
    • PROTEIN-PHOSPHORYLATION
    • SERINE/THREONINE/TYROSINE PHOSPHOPROTEOME

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