Comparison of MTT and ATP-based assays for the measurement of viable cell number

Russell D. Petty, Lesley A. Sutherland, Elizabeth M. Hunter, Ian A. Cree (Lead / Corresponding author)

    Research output: Contribution to journalArticle

    173 Citations (Scopus)

    Abstract

    Cell viability assays are widely used to assess the effect chemotherapeutic drugs and other agents on cell lines and have shown promise for the prediction of tumour chemosensitivity. In this study we have compared two viability assays using Daudi and CCRF-CEM cell lines over a range of 1500-100,000 cells/well of a microplate. The ATP assay was able to detect the lower limit of 1563 cells/well with luminescence values at least 100 x background readings, while the MTT assay could not detect less than 25,000 cells/well above background readings. The ATP assay also showed better reproducibility and sensitivity when cells were grown in microtitre plates over several days, and is particularly useful for the measurement of viability with low cell numbers.

    Original languageEnglish
    Pages (from-to)29-34
    Number of pages6
    JournalJournal of Bioluminescence and Chemiluminescence
    Volume10
    Issue number1
    DOIs
    Publication statusPublished - Jan 1995

    Fingerprint

    Assays
    Cell Count
    Adenosine Triphosphate
    Cells
    Reading
    Cell Line
    Luminescence
    Cell Survival
    Tumors
    Pharmaceutical Preparations
    Neoplasms

    Keywords

    • Adenosine Triphosphate
    • Animals
    • Beetles
    • Cell Count
    • Cell Death
    • Cell Line
    • Cell Survival
    • Coloring Agents
    • Humans
    • Luciferases
    • Luminescent Measurements
    • Sensitivity and Specificity
    • Tetrazolium Salts
    • Thiazoles
    • Tumor Cells, Cultured

    Cite this

    Petty, Russell D. ; Sutherland, Lesley A. ; Hunter, Elizabeth M. ; Cree, Ian A. / Comparison of MTT and ATP-based assays for the measurement of viable cell number. In: Journal of Bioluminescence and Chemiluminescence. 1995 ; Vol. 10, No. 1. pp. 29-34.
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    title = "Comparison of MTT and ATP-based assays for the measurement of viable cell number",
    abstract = "Cell viability assays are widely used to assess the effect chemotherapeutic drugs and other agents on cell lines and have shown promise for the prediction of tumour chemosensitivity. In this study we have compared two viability assays using Daudi and CCRF-CEM cell lines over a range of 1500-100,000 cells/well of a microplate. The ATP assay was able to detect the lower limit of 1563 cells/well with luminescence values at least 100 x background readings, while the MTT assay could not detect less than 25,000 cells/well above background readings. The ATP assay also showed better reproducibility and sensitivity when cells were grown in microtitre plates over several days, and is particularly useful for the measurement of viability with low cell numbers.",
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    Comparison of MTT and ATP-based assays for the measurement of viable cell number. / Petty, Russell D.; Sutherland, Lesley A.; Hunter, Elizabeth M.; Cree, Ian A. (Lead / Corresponding author).

    In: Journal of Bioluminescence and Chemiluminescence, Vol. 10, No. 1, 01.1995, p. 29-34.

    Research output: Contribution to journalArticle

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    T1 - Comparison of MTT and ATP-based assays for the measurement of viable cell number

    AU - Petty, Russell D.

    AU - Sutherland, Lesley A.

    AU - Hunter, Elizabeth M.

    AU - Cree, Ian A.

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    N2 - Cell viability assays are widely used to assess the effect chemotherapeutic drugs and other agents on cell lines and have shown promise for the prediction of tumour chemosensitivity. In this study we have compared two viability assays using Daudi and CCRF-CEM cell lines over a range of 1500-100,000 cells/well of a microplate. The ATP assay was able to detect the lower limit of 1563 cells/well with luminescence values at least 100 x background readings, while the MTT assay could not detect less than 25,000 cells/well above background readings. The ATP assay also showed better reproducibility and sensitivity when cells were grown in microtitre plates over several days, and is particularly useful for the measurement of viability with low cell numbers.

    AB - Cell viability assays are widely used to assess the effect chemotherapeutic drugs and other agents on cell lines and have shown promise for the prediction of tumour chemosensitivity. In this study we have compared two viability assays using Daudi and CCRF-CEM cell lines over a range of 1500-100,000 cells/well of a microplate. The ATP assay was able to detect the lower limit of 1563 cells/well with luminescence values at least 100 x background readings, while the MTT assay could not detect less than 25,000 cells/well above background readings. The ATP assay also showed better reproducibility and sensitivity when cells were grown in microtitre plates over several days, and is particularly useful for the measurement of viability with low cell numbers.

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    KW - Coloring Agents

    KW - Humans

    KW - Luciferases

    KW - Luminescent Measurements

    KW - Sensitivity and Specificity

    KW - Tetrazolium Salts

    KW - Thiazoles

    KW - Tumor Cells, Cultured

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