Complete structure of the glycan of lipopeptidophosphoglycan from Trypanosoma cruzi epimastigotes

Rosa M. de Lederkremer; Carlos Lima; Maria I. Ramirez; Michael A. J. Ferguson; Steve W. Homans; Jane Thomas-Oates

Complete structure of the glycan of lipopeptidophosphoglycan from Trypanosoma cruzi epimastigotes

Rosa M. de Lederkremer
, Carlos Lima
, Maria I. Ramirez
, Michael A. J. Ferguson
, Steve W. Homans
, Jane Thomas-Oates

Research output: Contribution to journal › Article › peer-review

Abstract

The lipopeptidophosphoglycan is the major cell surface glycoconjugate of the epimastigote forms of the parasitic protozoan Trypanosoma cruzi. A detailed partial structure for this molecule has been reported (Previato, J.O., Gorin, P.A.J., Mazurek, M., Xavier, M.T., Fournet, B., Wieruszesk, J.M., and Mendonca-Previato, L. (1990) J. Biol. Chem. 265, 2518-2526). In this study, we complete the primary structure assignments and describe the microheterogeneity found in the lipopeptidophosphoglycan glycan, using a combination of ¹H and ³¹P NMR, fast atom bombardment mass spectrometry, methylation linkage analysis, and exoglycosidase sequencing. The lipopeptidophosphoglycan is a glycosylated inositol-phosphoceramide with striking homology to glycosylphosphatidylinositol membrane anchors found attached to a wide variety of membrane anchors found attached to a wide variety of plasma membrane proteins throughout the eukaryotes.

Original language	English
Pages (from-to)	23670-23675
Number of pages	6
Journal	Journal of Biological Chemistry
Volume	266
Issue number	35
Publication status	Published - 15 Dec 1991

Access to Document

http://www.jbc.org/content/266/35/23670.abstract

Cite this

@article{c0afee4501fa4e2a80182f3bce397eb6,

title = "Complete structure of the glycan of lipopeptidophosphoglycan from Trypanosoma cruzi epimastigotes",

abstract = "The lipopeptidophosphoglycan is the major cell surface glycoconjugate of the epimastigote forms of the parasitic protozoan Trypanosoma cruzi. A detailed partial structure for this molecule has been reported (Previato, J.O., Gorin, P.A.J., Mazurek, M., Xavier, M.T., Fournet, B., Wieruszesk, J.M., and Mendonca-Previato, L. (1990) J. Biol. Chem. 265, 2518-2526). In this study, we complete the primary structure assignments and describe the microheterogeneity found in the lipopeptidophosphoglycan glycan, using a combination of 1H and 31P NMR, fast atom bombardment mass spectrometry, methylation linkage analysis, and exoglycosidase sequencing. The lipopeptidophosphoglycan is a glycosylated inositol-phosphoceramide with striking homology to glycosylphosphatidylinositol membrane anchors found attached to a wide variety of membrane anchors found attached to a wide variety of plasma membrane proteins throughout the eukaryotes.",

author = "\{de Lederkremer\}, \{Rosa M.\} and Carlos Lima and Ramirez, \{Maria I.\} and Ferguson, \{Michael A. J.\} and Homans, \{Steve W.\} and Jane Thomas-Oates",

year = "1991",

month = dec,

day = "15",

language = "English",

volume = "266",

pages = "23670--23675",

journal = "Journal of Biological Chemistry",

issn = "0021-9258",

publisher = "American Society for Biochemistry and Molecular Biology Inc.",

number = "35",

}

TY - JOUR

T1 - Complete structure of the glycan of lipopeptidophosphoglycan from Trypanosoma cruzi epimastigotes

AU - de Lederkremer, Rosa M.

AU - Lima, Carlos

AU - Ramirez, Maria I.

AU - Ferguson, Michael A. J.

AU - Homans, Steve W.

AU - Thomas-Oates, Jane

PY - 1991/12/15

Y1 - 1991/12/15

N2 - The lipopeptidophosphoglycan is the major cell surface glycoconjugate of the epimastigote forms of the parasitic protozoan Trypanosoma cruzi. A detailed partial structure for this molecule has been reported (Previato, J.O., Gorin, P.A.J., Mazurek, M., Xavier, M.T., Fournet, B., Wieruszesk, J.M., and Mendonca-Previato, L. (1990) J. Biol. Chem. 265, 2518-2526). In this study, we complete the primary structure assignments and describe the microheterogeneity found in the lipopeptidophosphoglycan glycan, using a combination of 1H and 31P NMR, fast atom bombardment mass spectrometry, methylation linkage analysis, and exoglycosidase sequencing. The lipopeptidophosphoglycan is a glycosylated inositol-phosphoceramide with striking homology to glycosylphosphatidylinositol membrane anchors found attached to a wide variety of membrane anchors found attached to a wide variety of plasma membrane proteins throughout the eukaryotes.

AB - The lipopeptidophosphoglycan is the major cell surface glycoconjugate of the epimastigote forms of the parasitic protozoan Trypanosoma cruzi. A detailed partial structure for this molecule has been reported (Previato, J.O., Gorin, P.A.J., Mazurek, M., Xavier, M.T., Fournet, B., Wieruszesk, J.M., and Mendonca-Previato, L. (1990) J. Biol. Chem. 265, 2518-2526). In this study, we complete the primary structure assignments and describe the microheterogeneity found in the lipopeptidophosphoglycan glycan, using a combination of 1H and 31P NMR, fast atom bombardment mass spectrometry, methylation linkage analysis, and exoglycosidase sequencing. The lipopeptidophosphoglycan is a glycosylated inositol-phosphoceramide with striking homology to glycosylphosphatidylinositol membrane anchors found attached to a wide variety of membrane anchors found attached to a wide variety of plasma membrane proteins throughout the eukaryotes.

UR - https://www.scopus.com/pages/publications/0026334201

M3 - Article

SN - 0021-9258

VL - 266

SP - 23670

EP - 23675

JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

IS - 35

ER -

Complete structure of the glycan of lipopeptidophosphoglycan from Trypanosoma cruzi epimastigotes

Abstract

Access to Document

Other files and links

Fingerprint

Cite this