Projects per year
Abstract
Trypanosomatids are parasitic protozoa with a significant burden on human health. African and American trypanosomes are causative agents of Nagana and Chagas disease respectively, and separated 300 million years ago. These parasites have highly distinct life cycles, pathologies, transmission strategies and surface proteomes, being dominated by the variant surface glycoprotein (African) or mucins (American) respectively. In African trypanosomes clathrin-mediated trafficking is responsible for endocytosis and post-Golgi transport, with several mechanistic aspects distinct from higher organisms. Using clathrin light chain (TcCLC) and EpsinR (TcEpsinR) as affinity handles, we identified candidate clathrin-associated proteins (CAPs) in Trypanosoma cruzi; the cohort includes orthologs of many proteins known to mediate vesicle trafficking, but significantly not the AP-2 adaptor complex. Several trypanosome-specific proteins common with African trypanosomes, were also identified. Fluorescence microscopy revealed localisations for TcEpsinR, TcCLC and TcCHC at the posterior region of trypomastigote cells, coincident with the flagellar pocket and Golgi apparatus. These data provide the first systematic analysis of clathrin-mediated trafficking in T. cruzi, allowing comparison between protein cohorts and other trypanosomes and also suggest that clathrin trafficking in at least some life stages of T. cruzi may be AP-2-independent.
Original language | English |
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Article number | 31212 |
Pages (from-to) | 1-12 |
Number of pages | 12 |
Journal | Scientific Reports |
Volume | 6 |
DOIs | |
Publication status | Published - 9 Aug 2016 |
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Dive into the research topics of 'Conservation and divergence within the clathrin interactome of Trypanosoma cruzi'. Together they form a unique fingerprint.Projects
- 1 Finished
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Targeting the Surface PRoteome of Trypanosoma Cruzi (Newton001) (Joint with Federal University of Sao Paulo, Brazil)
Field, M. (Investigator) & Horn, D. (Investigator)
1/01/15 → 31/12/15
Project: Research