Conservation of targeting but divergence in function and quality control of peroxisomal ABC transporters: An analysis using cross-kingdom expression

Xuebin Zhang, Carine De Marcos Lousa, Nellie Schutte-Lensink, Rob Ofman, Ronald J. Wanders, Stephen A. Baldwin, Alison Baker, Stephan Kemp, Frederica L. Theodoulou (Lead / Corresponding author)

Research output: Contribution to journalArticlepeer-review

34 Citations (Scopus)
27 Downloads (Pure)

Abstract

ABC (ATP-binding cassette) subfamily D transporters are found in all eukaryotic kingdoms and are known to play essential roles in mammals and plants; however, their number, organization and physiological contexts differ. Via cross-kingdom expression experiments, we have explored the conservation of targeting, protein stability and function between mammalian and plant ABCD transporters. When expressed in tobacco epidermal cells, the mammalian ABCD proteins ALDP (adrenoleukodystrophy protein), ALDR (adrenoleukodystrophy-related protein) and PMP70 (70 kDa peroxisomal membrane protein) targeted faithfully to peroxisomes and P70R (PMP70-related protein) targeted to the ER (endoplasmic reticulum), as in the native host. The Arabidopsis thaliana peroxin AtPex19-1 interacted with human peroxisomal ABC transporters both in vivo and in vitro, providing an explanation for the fidelity of targeting. The fate of X-linked adrenoleukodystrophy disease-related mutants differed between fibroblasts and plant cells. In fibroblasts, levels of ALDP in some 'protein-absent' mutantswere increased by low-temperature culture, in some cases restoring function. In contrast, all mutant ALDP proteins examined were stable and correctly targeted in plant cells, regardless of their fate in fibroblasts.ALDR complemented the seed germination defect of the Arabidopsis cts-1 mutant which lacks the peroxisomal ABCD transporter CTS (Comatose), but neither ALDR nor ALDP was able to rescue the defect in fatty acid β-oxidation in establishing seedlings. Taken together, our results indicate that the mechanism for trafficking of peroxisomal membrane proteins is shared between plants and mammals, but suggest differences in the sensing and turnover of mutant ABC transporter proteins and differences in substrate specificity and/or function.

Original languageEnglish
Pages (from-to)547-557
Number of pages11
JournalBiochemical Journal
Volume436
Issue number3
Early online date11 Apr 2011
DOIs
Publication statusPublished - 15 Jun 2011

Keywords

  • 70 kDa peroxisomal membrane protein (PMP70)
  • Adrenoleukodystrophy protein (ALDP)
  • ATP-binding cassette transporter (ABC transporter)
  • Comatose (CTS)
  • Peroxisome
  • Pex19
  • X-linked adrenoleukodystrophy (X-ALD)

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Fingerprint

Dive into the research topics of 'Conservation of targeting but divergence in function and quality control of peroxisomal ABC transporters: An analysis using cross-kingdom expression'. Together they form a unique fingerprint.

Cite this