Conserved network of proteins essential for bacterial viability

Jennifer I. Handford, Berengere Ize, Grant Buchanan, Gareth P. Butland, Jack Greenblatt, Andrew Emili, Tracy Palmer

    Research output: Contribution to journalArticlepeer-review

    59 Citations (Scopus)

    Abstract

    The yjeE, yeaZ, and ygjD genes are highly conserved in the genomes of eubacteria, and ygjD orthologs are also found throughout the Archaea and eukaryotes. In this study, we have constructed conditional expression strains for each of these genes in the model organism Escherichia coli K12. We show that each gene is essential for the viability of E. coli under laboratory growth conditions. Growth of the conditional strains under nonpermissive conditions results in dramatic changes in cell ultrastructure. Deliberate repression of the expression of yeaZ results in cells with highly condensed nucleoids, while repression of yjeE and ygjD expression results in at least a proportion of very enlarged cells with an unusual peripheral distribution of DNA. Each of the three conditional expression strains can be complemented by multicopy clones harboring the rstA gene, which encodes a two-component-system response regulator, strongly suggesting that these proteins are involved in the same essential cellular pathway. The results of bacterial two-hybrid experiments show that YeaZ can interact with both YjeE and YgjD but that YgjD is the preferred interaction partner. The results of in vitro experiments indicate that YeaZ mediates the proteolysis of YgjD, suggesting that YeaZ and YjeE act as regulators to control the activity of this protein. Our results are consistent with these proteins forming a link between DNA metabolism and cell division.

    Original languageEnglish
    Pages (from-to)4732-4749
    Number of pages18
    JournalJournal of Bacteriology
    Volume191
    Issue number15
    DOIs
    Publication statusPublished - Aug 2009

    Keywords

    • HAEMOLYTICA A1 GLYCOPROTEASE
    • ESCHERICHIA-COLI
    • ESSENTIAL GENES
    • BACILLUS-SUBTILIS
    • UNKNOWN FUNCTION
    • TRANSLOCATION PATHWAY
    • NUCLEOID CONDENSATION
    • 2-HYBRID SYSTEM
    • KEOPS COMPLEX
    • IN-VITRO

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