Constitutive endocytosis and recycling of major histocompatibility complex class II glycoproteins in human B-lymphoblastoid cells

P. A. Reid, C. Watts

    Research output: Contribution to journalArticlepeer-review

    28 Citations (Scopus)

    Abstract

    Cleavable cell surface radiolabelling reagents were used to measure the endocytosis and recycling of class II major histocompatibility complex (MHC) glycoproteins in human B-lymphoblastoid cells. It is shown that mature class II αβ dimers on the cell surface are constitutively endocytosed and that recycling can be demonstrated even from small endosomal pools. Endocytosis was blocked when cellular ATP levels were depleted or when clathrin polymerization was inhibited, implicating clathrin-coated pits in the endocytic process. Taken together with earlier studies, these results suggest that mature class II MHC molecules are constitutively endocytosed and recycled from acidic peripheral endosomes which may enhance their capacity to bind and present T cell epitopes which do not require processing.

    Original languageEnglish
    Pages (from-to)539-542
    Number of pages4
    JournalImmunology
    Volume77
    Issue number4
    Publication statusPublished - Dec 1992

    ASJC Scopus subject areas

    • Immunology and Allergy
    • Immunology

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