Context-Specific Requirement of Forty-Four Two-Component Loci in Pseudomonas aeruginosa Swarming

Ameen M. Kollaran, Shubham Joge, Harshitha S. Kotian, Divakar Badal, Deep Prakash, Ayushi Mishra, Manoj Varma, Varsha Singh (Lead / Corresponding author)

Research output: Contribution to journalArticlepeer-review

28 Citations (Scopus)
2 Downloads (Pure)

Abstract

Swarming in Pseudomonas aeruginosa is a coordinated movement o bacteria over semisolid surfaces (0.5%–0.7%agar). On soft agar, P. aeruginosa exhibits a dendritic swarm pattern, with multiple levels of branching. However, the swarm patterns typically vary depending upon the experimental design. In the present study, we show that the pattern characteristics of P. aeruginosa swarm are highly environmen dependent. We define several quantifiable, macroscale features of the swarm to study the plasticity of the swarm, observed across different nutrient formulations. Furthermore, through a targeted screen of 113 two-component system (TCS) loci of the P. aeruginosa strain PA 14, we show that forty-four TCS genes regulate swarming in PA14 in a contextual fashion. However, only four TCS genes—fleR, fleS, gacS, and PA14_59770—were found essential for swarming. Notably, many swarming-defective TCS mutants were found highly efficient in biofilm formation, indicating opposing roles for many TCS loci.
Original languageEnglish
Pages (from-to)305-317
Number of pages13
JournaliScience
Volume13
Early online date1 Mar 2019
DOIs
Publication statusPublished - 29 Mar 2019

Keywords

  • Biological Sciences
  • Microbiology
  • Pathogenic Organism

ASJC Scopus subject areas

  • General

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