TY - JOUR
T1 - Context-Specific Requirement of Forty-Four Two-Component Loci in Pseudomonas aeruginosa Swarming
AU - Kollaran, Ameen M.
AU - Joge, Shubham
AU - Kotian, Harshitha S.
AU - Badal, Divakar
AU - Prakash, Deep
AU - Mishra, Ayushi
AU - Varma, Manoj
AU - Singh, Varsha
N1 - Publisher Copyright:
© 2019 The Authors
PY - 2019/3/29
Y1 - 2019/3/29
N2 - Swarming in Pseudomonas aeruginosa is a coordinated movement o bacteria over semisolid surfaces (0.5%–0.7%agar). On soft agar, P. aeruginosa exhibits a dendritic swarm pattern, with multiple levels of branching. However, the swarm patterns typically vary depending upon the experimental design. In the present study, we show that the pattern characteristics of P. aeruginosa swarm are highly environmen dependent. We define several quantifiable, macroscale features of the swarm to study the plasticity of the swarm, observed across different nutrient formulations. Furthermore, through a targeted screen of 113 two-component system (TCS) loci of the P. aeruginosa strain PA 14, we show that forty-four TCS genes regulate swarming in PA14 in a contextual fashion. However, only four TCS genes—fleR, fleS, gacS, and PA14_59770—were found essential for swarming. Notably, many swarming-defective TCS mutants were found highly efficient in biofilm formation, indicating opposing roles for many TCS loci.
AB - Swarming in Pseudomonas aeruginosa is a coordinated movement o bacteria over semisolid surfaces (0.5%–0.7%agar). On soft agar, P. aeruginosa exhibits a dendritic swarm pattern, with multiple levels of branching. However, the swarm patterns typically vary depending upon the experimental design. In the present study, we show that the pattern characteristics of P. aeruginosa swarm are highly environmen dependent. We define several quantifiable, macroscale features of the swarm to study the plasticity of the swarm, observed across different nutrient formulations. Furthermore, through a targeted screen of 113 two-component system (TCS) loci of the P. aeruginosa strain PA 14, we show that forty-four TCS genes regulate swarming in PA14 in a contextual fashion. However, only four TCS genes—fleR, fleS, gacS, and PA14_59770—were found essential for swarming. Notably, many swarming-defective TCS mutants were found highly efficient in biofilm formation, indicating opposing roles for many TCS loci.
KW - Biological Sciences
KW - Microbiology
KW - Pathogenic Organism
UR - http://www.scopus.com/inward/record.url?scp=85066239826&partnerID=8YFLogxK
U2 - 10.1016/j.isci.2019.02.028
DO - 10.1016/j.isci.2019.02.028
M3 - Article
AN - SCOPUS:85066239826
SN - 2589-0042
VL - 13
SP - 305
EP - 317
JO - iScience
JF - iScience
ER -