Projects per year
To ensure proper segregation during mitosis, chromosomes must be efficiently captured by spindle microtubules and subsequently aligned on the mitotic spindle. The efficacy of chromosome interaction with the spindle can be influenced by how widely chromosomes are scattered in space. Here, we quantify chromosome-scattering volume (CSV) and find that it is reduced soon after nuclear envelope breakdown (NEBD) in human cells. The CSV reduction occurs primarily independently of microtubules and is therefore not an outcome of interactions between chromosomes and the spindle. We find that, prior to NEBD, an acto-myosin network is assembled in a LINC complex-dependent manner on the cytoplasmic surface of the nuclear envelope. This acto-myosin network remains on nuclear envelope remnants soon after NEBD, and its myosin-II-mediated contraction reduces CSV and facilitates timely chromosome congression and correct segregation. Thus, we find a novel mechanism that positions chromosomes in early mitosis to ensure efficient and correct chromosome-spindle interactions.
|Number of pages||21|
|Early online date||2 Jul 2019|
|Publication status||Published - 16 Jul 2019|
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- 4 Finished
Advancing Tissue Imaging Capabilities with Airyscan Confocal Microscopy (Multiuser Equipment Grant)
Ganley, I., Januschke, J., Muqit, M., Storey, K., Swedlow, J. & Tanaka, T.
26/09/17 → 1/01/19
Strategic Award: Wellcome Trust Technology Platform
Blow, J., Lamond, A. & Owen-Hughes, T.
1/01/13 → 30/09/18
Dynamics of Fundamental Cellular Processes by Live Cell and Tissue Imaging
MacDonald, M., McGloin, D., McKenna, S., Storey, K., Swedlow, J. & Weijer, K.
1/01/13 → 31/12/17
- Gene Regulation and Expression - Professor of Cell and Molecular Biology