Contribution of casein kinase 2 and spleen tyrosine kinase to CFTR trafficking and protein kinase A-induced activity

Simao Luz, Patthara Kongsuphol, Ana Isabel Mendes, Francisco Romeiras, Marisa Sousa, Rainer Schreiber, Paulo Matos, Peter Jordan, Anil Mehta, Margarida D. Amaral, Karl Kunzelmann, Carlos M. Farinha

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    37 Citations (Scopus)


    Previously, the pleiotropic "master kinase" casein kinase 2 (CK2) was shown to interact with CFTR, the protein responsible for cystic fibrosis (CF). Moreover, CK2 inhibition abolished CFTR conductance in cell-attached membrane patches, native epithelial ducts, and Xenopus oocytes. CFTR possesses two CK2 phosphorylation sites (S422 and T1471), with unclear impact on its processing and trafficking. Here, we investigated the effects of mutating these CK2 sites on CFTR abundance, maturation, and degradation coupled to effects on ion channel activity and surface expression. We report that CK2 inhibition significantly decreased processing of wild-type (wt) CFTR, with no effect on F508del CFTR. Eliminating phosphorylation at S422 and T1471 revealed antagonistic roles in CFTR trafficking: S422 activation versus T1471 inhibition, as evidenced by a severe trafficking defect for the T1471D mutant. Notably, mutation of Y512, a consensus sequence for the spleen tyrosine kinase (SYK) possibly acting in a CK2 context adjacent to the common CF-causing defect F508del, had a strong effect on both maturation and CFTR currents, allowing the identification of this kinase as a novel regulator of CFTR. These results reinforce the importance of CK2 and the S422 and T1471 residues for regulation of CFTR and uncover a novel regulation of CFTR by SYK, a recognized controller of inflammation.
    Original languageEnglish
    Pages (from-to)4392-4404
    Number of pages13
    JournalMolecular and Cellular Biology
    Issue number22
    Publication statusPublished - Nov 2011


    • Animals
    • Intracellular Signaling Peptides and Proteins
    • Ion Channels
    • Protein-Tyrosine Kinases
    • Mice
    • Casein Kinase II
    • Xenopus laevis
    • Phosphorylation
    • Cystic Fibrosis Transmembrane Conductance Regulator
    • Mice, Inbred C57BL
    • Cyclic AMP-Dependent Protein Kinases
    • Mutation
    • Cricetinae
    • Protein Transport


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