Control of inhibition by the direct action of cannabinoids on GABAA receptors

Tatiana Golovko, Rogier Min, Natalia Lozovaya, Caroline Falconer, Natalia Yatsenko, Timur Tsintsadze, Vera Tsintsadze, Catherine Ledent, Robert J. Harvey, Delia Belelli, Jeremy J. Lambert, Andrei Rozov, Nail Burnashev (Lead / Corresponding author)

    Research output: Contribution to journalArticle

    13 Citations (Scopus)

    Abstract

    Cannabinoids are known to regulate inhibitory synaptic transmission via activation of presynaptic G protein-coupled cannabinoid CB1 receptors (CB1Rs). Additionally, recent studies suggest that cannabinoids can also directly interact with recombinant GABAA receptors (GABAARs), potentiating currents activated by micromolar concentrations of γ-aminobutyric acid (GABA). However, the impact of this direct interaction on GABAergic inhibition in central nervous system is unknown. Here we report that currents mediated by recombinant GABAARs activated by high (synaptic) concentrations of GABA as well as GABAergic inhibitory postsynaptic currents (IPSCs) at neocortical fast spiking (FS) interneuron to pyramidal neuron synapses are suppressed by exogenous and endogenous cannabinoids in a CB1R-independent manner. This IPSC suppression may account for disruption of inhibitory control of pyramidal neurons by FS interneurons. At FS interneuron to pyramidal neuron synapses, endocannabinoids induce synaptic low-pass filtering of GABAAR-mediated currents evoked by high-frequency stimulation. The CB1R-independent suppression of inhibition is synapse specific. It does not occur in CB1R containing hippocampal cholecystokinin-positive interneuron to pyramidal neuron synapses. Furthermore, in contrast to synaptic receptors, the activity of extrasynaptic GABAARs in neocortical pyramidal neurons is enhanced by cannabinoids in a CB1R-independent manner. Thus, cannabinoids directly interact differentially with synaptic and extrasynaptic GABAARs, providing a potent novel context-dependent mechanism for regulation of inhibition.

    Original languageEnglish
    Pages (from-to)2440-2455
    Number of pages16
    JournalCerebral Cortex
    Volume25
    Issue number9
    Early online date18 Mar 2014
    DOIs
    Publication statusPublished - 1 Sep 2015

    Fingerprint

    Cannabinoid Receptor CB1
    Cannabinoids
    Pyramidal Cells
    GABA-A Receptors
    Interneurons
    Synapses
    Inhibitory Postsynaptic Potentials
    gamma-Aminobutyric Acid
    Aminobutyrates
    Endocannabinoids
    Neurotransmitter Receptor
    Cholecystokinin
    GTP-Binding Proteins
    Synaptic Transmission
    Central Nervous System

    Keywords

    • Cb receptors
    • DSI
    • Endocannabinoids
    • Fast-spiking interneurons
    • Inhibitory synaptic transmission
    • Neocortex

    Cite this

    Golovko, T., Min, R., Lozovaya, N., Falconer, C., Yatsenko, N., Tsintsadze, T., ... Burnashev, N. (2015). Control of inhibition by the direct action of cannabinoids on GABAA receptors. Cerebral Cortex, 25(9), 2440-2455. https://doi.org/10.1093/cercor/bhu045
    Golovko, Tatiana ; Min, Rogier ; Lozovaya, Natalia ; Falconer, Caroline ; Yatsenko, Natalia ; Tsintsadze, Timur ; Tsintsadze, Vera ; Ledent, Catherine ; Harvey, Robert J. ; Belelli, Delia ; Lambert, Jeremy J. ; Rozov, Andrei ; Burnashev, Nail. / Control of inhibition by the direct action of cannabinoids on GABAA receptors. In: Cerebral Cortex. 2015 ; Vol. 25, No. 9. pp. 2440-2455.
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    abstract = "Cannabinoids are known to regulate inhibitory synaptic transmission via activation of presynaptic G protein-coupled cannabinoid CB1 receptors (CB1Rs). Additionally, recent studies suggest that cannabinoids can also directly interact with recombinant GABAA receptors (GABAARs), potentiating currents activated by micromolar concentrations of γ-aminobutyric acid (GABA). However, the impact of this direct interaction on GABAergic inhibition in central nervous system is unknown. Here we report that currents mediated by recombinant GABAARs activated by high (synaptic) concentrations of GABA as well as GABAergic inhibitory postsynaptic currents (IPSCs) at neocortical fast spiking (FS) interneuron to pyramidal neuron synapses are suppressed by exogenous and endogenous cannabinoids in a CB1R-independent manner. This IPSC suppression may account for disruption of inhibitory control of pyramidal neurons by FS interneurons. At FS interneuron to pyramidal neuron synapses, endocannabinoids induce synaptic low-pass filtering of GABAAR-mediated currents evoked by high-frequency stimulation. The CB1R-independent suppression of inhibition is synapse specific. It does not occur in CB1R containing hippocampal cholecystokinin-positive interneuron to pyramidal neuron synapses. Furthermore, in contrast to synaptic receptors, the activity of extrasynaptic GABAARs in neocortical pyramidal neurons is enhanced by cannabinoids in a CB1R-independent manner. Thus, cannabinoids directly interact differentially with synaptic and extrasynaptic GABAARs, providing a potent novel context-dependent mechanism for regulation of inhibition.",
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    Golovko, T, Min, R, Lozovaya, N, Falconer, C, Yatsenko, N, Tsintsadze, T, Tsintsadze, V, Ledent, C, Harvey, RJ, Belelli, D, Lambert, JJ, Rozov, A & Burnashev, N 2015, 'Control of inhibition by the direct action of cannabinoids on GABAA receptors', Cerebral Cortex, vol. 25, no. 9, pp. 2440-2455. https://doi.org/10.1093/cercor/bhu045

    Control of inhibition by the direct action of cannabinoids on GABAA receptors. / Golovko, Tatiana; Min, Rogier; Lozovaya, Natalia; Falconer, Caroline; Yatsenko, Natalia; Tsintsadze, Timur; Tsintsadze, Vera; Ledent, Catherine; Harvey, Robert J.; Belelli, Delia; Lambert, Jeremy J.; Rozov, Andrei; Burnashev, Nail (Lead / Corresponding author).

    In: Cerebral Cortex, Vol. 25, No. 9, 01.09.2015, p. 2440-2455.

    Research output: Contribution to journalArticle

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    AU - Min, Rogier

    AU - Lozovaya, Natalia

    AU - Falconer, Caroline

    AU - Yatsenko, Natalia

    AU - Tsintsadze, Timur

    AU - Tsintsadze, Vera

    AU - Ledent, Catherine

    AU - Harvey, Robert J.

    AU - Belelli, Delia

    AU - Lambert, Jeremy J.

    AU - Rozov, Andrei

    AU - Burnashev, Nail

    N1 - This work was supported by International Brain Research Organization (IBRO), Research grant of the Netherlands Organisation for Scientific Research (NWO), and Marie Curie IIF 237781 “CANNABISTARG” grants to N.L. and Fondation pour la Recherche Médicale (FRM)—“Nouvelle Equipe’ grant to N.B.. The Department of Integrative Neurophysiology was financially supported by NeuroBsik (www.neurobsik.nl). Experiments on the α2−/− mouse were supported by the MRC (G1000008) to J.J.L. and D.B.).

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    N2 - Cannabinoids are known to regulate inhibitory synaptic transmission via activation of presynaptic G protein-coupled cannabinoid CB1 receptors (CB1Rs). Additionally, recent studies suggest that cannabinoids can also directly interact with recombinant GABAA receptors (GABAARs), potentiating currents activated by micromolar concentrations of γ-aminobutyric acid (GABA). However, the impact of this direct interaction on GABAergic inhibition in central nervous system is unknown. Here we report that currents mediated by recombinant GABAARs activated by high (synaptic) concentrations of GABA as well as GABAergic inhibitory postsynaptic currents (IPSCs) at neocortical fast spiking (FS) interneuron to pyramidal neuron synapses are suppressed by exogenous and endogenous cannabinoids in a CB1R-independent manner. This IPSC suppression may account for disruption of inhibitory control of pyramidal neurons by FS interneurons. At FS interneuron to pyramidal neuron synapses, endocannabinoids induce synaptic low-pass filtering of GABAAR-mediated currents evoked by high-frequency stimulation. The CB1R-independent suppression of inhibition is synapse specific. It does not occur in CB1R containing hippocampal cholecystokinin-positive interneuron to pyramidal neuron synapses. Furthermore, in contrast to synaptic receptors, the activity of extrasynaptic GABAARs in neocortical pyramidal neurons is enhanced by cannabinoids in a CB1R-independent manner. Thus, cannabinoids directly interact differentially with synaptic and extrasynaptic GABAARs, providing a potent novel context-dependent mechanism for regulation of inhibition.

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    Golovko T, Min R, Lozovaya N, Falconer C, Yatsenko N, Tsintsadze T et al. Control of inhibition by the direct action of cannabinoids on GABAA receptors. Cerebral Cortex. 2015 Sep 1;25(9):2440-2455. https://doi.org/10.1093/cercor/bhu045