Cooperative Control of Holliday Junction Resolution and DNA Repair by the SLX1 and MUS81-EME1 Nucleases

Dennis Castor, Nidhi Nair, Anne-Cécile Déclais, Christophe Lachaud, Rachel Toth, Thomas J. Macartney, David M.J. Lilley, J. Simon C. Arthur, John Rouse (Lead / Corresponding author)

    Research output: Contribution to journalArticle

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    Abstract

    Holliday junctions (HJs) are X-shaped DNA structures that arise during homologous recombination, which must be removed to enable chromosome segregation. The SLX1 and MUS81-EME1 nucleases can both process HJs in vitro, and they bind in close proximity on the SLX4 scaffold, hinting at possible cooperation. However, the cellular roles of mammalian SLX1 are not yet known. Here, we use mouse genetics and structure function analysis to investigate SLX1 function. Disrupting the murine Slx1 and Slx4 genes revealed that they are essential for HJ resolution in mitotic cells. Moreover, SLX1 and MUS81-EME1 act together to resolve HJs in a manner that requires tethering to SLX4. We also show that SLX1, like MUS81-EME1, is required for repair of DNA interstrand crosslinks, but this role appears to be independent of HJ cleavage, at least in mouse cells. These findings shed light on HJ resolution in mammals and on maintenance of genome stability. "Resolution of Holliday junctions in mouse cells requires the SLX1 nuclease"SLX1 acts cooperatively with MUS81-EME1 in HJ resolution and ICL repair"Mutations in SLX4 that prevent it binding to SLX1 and MUS81-EME1 abolish HJ resolution"DNA substrates of SLX1 and MUS81-EME1 in ICL repair appear to be different from HJs
    Original languageEnglish
    Pages (from-to)221-233
    Number of pages13
    JournalMolecular Cell
    Volume52
    Issue number2
    DOIs
    Publication statusPublished - 24 Oct 2013

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    Cruciform DNA
    DNA Repair
    Chromosome Segregation
    Genetic Structures
    Genomic Instability
    Homologous Recombination
    Essential Genes
    Mammals
    Maintenance

    Cite this

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    title = "Cooperative Control of Holliday Junction Resolution and DNA Repair by the SLX1 and MUS81-EME1 Nucleases",
    abstract = "Holliday junctions (HJs) are X-shaped DNA structures that arise during homologous recombination, which must be removed to enable chromosome segregation. The SLX1 and MUS81-EME1 nucleases can both process HJs in vitro, and they bind in close proximity on the SLX4 scaffold, hinting at possible cooperation. However, the cellular roles of mammalian SLX1 are not yet known. Here, we use mouse genetics and structure function analysis to investigate SLX1 function. Disrupting the murine Slx1 and Slx4 genes revealed that they are essential for HJ resolution in mitotic cells. Moreover, SLX1 and MUS81-EME1 act together to resolve HJs in a manner that requires tethering to SLX4. We also show that SLX1, like MUS81-EME1, is required for repair of DNA interstrand crosslinks, but this role appears to be independent of HJ cleavage, at least in mouse cells. These findings shed light on HJ resolution in mammals and on maintenance of genome stability. {"}Resolution of Holliday junctions in mouse cells requires the SLX1 nuclease{"}SLX1 acts cooperatively with MUS81-EME1 in HJ resolution and ICL repair{"}Mutations in SLX4 that prevent it binding to SLX1 and MUS81-EME1 abolish HJ resolution{"}DNA substrates of SLX1 and MUS81-EME1 in ICL repair appear to be different from HJs",
    author = "Dennis Castor and Nidhi Nair and Anne-C{\'e}cile D{\'e}clais and Christophe Lachaud and Rachel Toth and Macartney, {Thomas J.} and Lilley, {David M.J.} and Arthur, {J. Simon C.} and John Rouse",
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    Cooperative Control of Holliday Junction Resolution and DNA Repair by the SLX1 and MUS81-EME1 Nucleases. / Castor, Dennis; Nair, Nidhi; Déclais, Anne-Cécile; Lachaud, Christophe; Toth, Rachel; Macartney, Thomas J.; Lilley, David M.J.; Arthur, J. Simon C.; Rouse, John (Lead / Corresponding author).

    In: Molecular Cell, Vol. 52, No. 2, 24.10.2013, p. 221-233.

    Research output: Contribution to journalArticle

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    AU - Castor, Dennis

    AU - Nair, Nidhi

    AU - Déclais, Anne-Cécile

    AU - Lachaud, Christophe

    AU - Toth, Rachel

    AU - Macartney, Thomas J.

    AU - Lilley, David M.J.

    AU - Arthur, J. Simon C.

    AU - Rouse, John

    PY - 2013/10/24

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    N2 - Holliday junctions (HJs) are X-shaped DNA structures that arise during homologous recombination, which must be removed to enable chromosome segregation. The SLX1 and MUS81-EME1 nucleases can both process HJs in vitro, and they bind in close proximity on the SLX4 scaffold, hinting at possible cooperation. However, the cellular roles of mammalian SLX1 are not yet known. Here, we use mouse genetics and structure function analysis to investigate SLX1 function. Disrupting the murine Slx1 and Slx4 genes revealed that they are essential for HJ resolution in mitotic cells. Moreover, SLX1 and MUS81-EME1 act together to resolve HJs in a manner that requires tethering to SLX4. We also show that SLX1, like MUS81-EME1, is required for repair of DNA interstrand crosslinks, but this role appears to be independent of HJ cleavage, at least in mouse cells. These findings shed light on HJ resolution in mammals and on maintenance of genome stability. "Resolution of Holliday junctions in mouse cells requires the SLX1 nuclease"SLX1 acts cooperatively with MUS81-EME1 in HJ resolution and ICL repair"Mutations in SLX4 that prevent it binding to SLX1 and MUS81-EME1 abolish HJ resolution"DNA substrates of SLX1 and MUS81-EME1 in ICL repair appear to be different from HJs

    AB - Holliday junctions (HJs) are X-shaped DNA structures that arise during homologous recombination, which must be removed to enable chromosome segregation. The SLX1 and MUS81-EME1 nucleases can both process HJs in vitro, and they bind in close proximity on the SLX4 scaffold, hinting at possible cooperation. However, the cellular roles of mammalian SLX1 are not yet known. Here, we use mouse genetics and structure function analysis to investigate SLX1 function. Disrupting the murine Slx1 and Slx4 genes revealed that they are essential for HJ resolution in mitotic cells. Moreover, SLX1 and MUS81-EME1 act together to resolve HJs in a manner that requires tethering to SLX4. We also show that SLX1, like MUS81-EME1, is required for repair of DNA interstrand crosslinks, but this role appears to be independent of HJ cleavage, at least in mouse cells. These findings shed light on HJ resolution in mammals and on maintenance of genome stability. "Resolution of Holliday junctions in mouse cells requires the SLX1 nuclease"SLX1 acts cooperatively with MUS81-EME1 in HJ resolution and ICL repair"Mutations in SLX4 that prevent it binding to SLX1 and MUS81-EME1 abolish HJ resolution"DNA substrates of SLX1 and MUS81-EME1 in ICL repair appear to be different from HJs

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