TY - JOUR
T1 - Copper uptake by Penicillium ochro-chloron
T2 - Influence of pH on toxicity and demonstration of energy-dependent copper influx using protoplasts
AU - Gadd, G. M.
AU - White, C.
PY - 1985/8/1
Y1 - 1985/8/1
N2 - The existence of energy-dependent copper influx was demonstrated in protoplasts of Penicillium ochro-chloron. Protoplasts and mycelium were tolerant of copper over the pH range 3·0 to 5·5 but sensitive at pH 6·0. Uptake of copper was approximately ten times greater at pH 6·0 than in the lower range. At pH 3·0, influx showed saturation kinetics with a half-maximal influx at an external Cu2+ concentration of 390 μ;M and a maximum influx rate of 22 nmol h−1 (107 cells)−1. Saturation kinetics were not observed at pH 6·0.
AB - The existence of energy-dependent copper influx was demonstrated in protoplasts of Penicillium ochro-chloron. Protoplasts and mycelium were tolerant of copper over the pH range 3·0 to 5·5 but sensitive at pH 6·0. Uptake of copper was approximately ten times greater at pH 6·0 than in the lower range. At pH 3·0, influx showed saturation kinetics with a half-maximal influx at an external Cu2+ concentration of 390 μ;M and a maximum influx rate of 22 nmol h−1 (107 cells)−1. Saturation kinetics were not observed at pH 6·0.
UR - http://www.scopus.com/inward/record.url?scp=0022344866&partnerID=8YFLogxK
U2 - 10.1099/00221287-131-8-1875
DO - 10.1099/00221287-131-8-1875
M3 - Article
AN - SCOPUS:0022344866
SN - 0022-1287
VL - 131
SP - 1875
EP - 1879
JO - Journal of General Microbiology
JF - Journal of General Microbiology
IS - 8
ER -