Copper uptake by yeast-like cells, hyphae, and chlamydospores of Aureobasidium pullulans

Geoffrey M. Gadd, Jonathan L. Mowll

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    81 Citations (Scopus)


    All cell types of Aureobasidium pullulans could bind copper to surfaces, the efficiency of binding being in the order chlamydospores >yeast-like cells >mycelium. Chlamydospores were the least sensitive cell type to copper and only yeast-like cells and mycelium exhibited a second phase of energy-dependent Cu2+ influx at nontoxic concentrations. This followed Michaelis-Menten kinetics and was inhibited at 4°C or in the presence of 2-deoxy-d-glucose, potassium cyanide, high Ca2+ concentrations, and the uncouplers carbonylcyanide-p-trifluoromethoxyphenylhydrazone and dinitrophenol, but was stimulated by nigericin. Concomitant with Cu2+ influx was K+ efflux, and two K+ ions were released for every Cu2+ taken up. Cu2+ influx was inhibited when intracellular K+ was low or when extracellular K+ was high. The results suggested that Cu2+ influx was driven by the membrane potential. There was Cu2+ efflux from loaded cells which was independent of the presence of glucose, and kinetic analysis showed that the cells acted as two-compartment systems. Most of the intracellular copper could be removed by washing the cells with nitrilotriacetic acid.

    Original languageEnglish
    Pages (from-to)0-40
    Number of pages41
    JournalExperimental Mycology
    Issue number3
    Publication statusPublished - Sept 1985


    • Aureobasidium pullulans
    • chlamydospores
    • copper binding
    • copper uptake
    • energy-dependent Cu influx
    • hyphae
    • yeast-like cells

    ASJC Scopus subject areas

    • Applied Microbiology and Biotechnology


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