Research output per year
Research output per year
Ana Pavić, Alexandra O.M. Holmes, Vincent L.G. Postis, Adrian Goldman
Research output: Contribution to journal › Comment/debate › peer-review
It has come to the attention of the authors that a citation for Figure 4 was omitted from the caption. The caption should read:
Figure 4. Predicted anion permeation pathway.
A GltPh monomer viewed from the trimerization interface. The residues R276 and M395 responsible for the anion selectivity are indicated. The orange circles represent 'snapshots' of the path of a chloride ion through the channel between the two domains. Part of the scaffold domain has been removed for clarity. Figure adapted from [49].
Additionally, some references were incorrectly cited in the second paragraph of the section 'Uncoupled Chloride Conductance'. The paragraph should read:
Initially, chloride permeation was proposed to occur along TM2 [47]. The Ser to Val mutation on TM2 (residue 65 in GltPh and 103 in EAAT1) strongly affected chloride permeation [8,47]. The GltPh structure in an intermediate OFC suggested that there was an aqueous cavity that might serve as a permeation pathway for anions [14]. Subsequent research has suggested that substrate transport and anion permeation proceed through two mutually exclusive pathways separated by a flexible wall domain and facilitated by the conformational changes [48,49]. The moving flexible wall is composed of several residues in HP1, HP2 hairpins and TM8 (Figure 4), the movement of which dictate the pathway [49]. Arg276 in HP1 and Met395, predicted to line the anion pore [49], were shown to exhibit a significant Cl-selectivity over Na+ in both simulations and experiments: Anion selectivity is impaired by the insertion of negatively charged side chains at specific positions. In contrast with previous research [48], mutations of Ser65 did not affect anion permeation. Presumably, therefore, Ser65 (Ser103) is part of the channel-opening mechanism but not of the permeation pathway. As the permeation pathway accounted for all known functional properties of EAAT/GltPh anion channels during simulations, it is the most likely mechanism of uncoupled chloride conductance.
Original language | English |
---|---|
Pages (from-to) | 1567 |
Number of pages | 1 |
Journal | Biochemical Society Transactions |
Volume | 47 |
Issue number | 5 |
DOIs |
|
Publication status | Published - 5 Sept 2019 |
Research output: Contribution to journal › Article › peer-review