Correlation between parotid saliva composition and dental caries using 31P-NMR and ICDAS score

Angela Rovera (Lead / Corresponding author), Guido Rovera, Ali Alzahrani, Mark Hector, Paul Anderson

    Research output: Contribution to journalArticle

    Abstract

    Objectives: The preservation of enamel mineral is influenced by the supersaturation of salivary secretions with respect to calcium phosphate salts. The aim was to measure the chemical environmental state of phosphate ions in a subject's parotid saliva, and to correlate this with their dental caries score, by means of 31P-Nuclear Magnetic Resonance Spectroscopy (31P-NMR) and the International Caries Detection and Assessment System (ICDAS).

    Design: Unilateral paraffin wax stimulated parotid saliva samples were collected from 21 healthy adult subjects using a Lashley cup. The flowrate was recorded during collection. Clinical caries scores of each subject were classified using the ICDAS score. The pH was recorded for each saliva sample. 31P-NMR spectra of each saliva sample were obtained to determine the phosphorus chemical environment. All the collected data were analysed by Pearson's correlation.

    Results: Parotid saliva flow rates were in the range from 0.07 to 0.56 ml/min. The pH varied from 5.9 to 7.6. Each 31P-NMR spectrum showed a single broad line with a chemical shift between 0.07 and 2.38 ppm. At neutral pH the maximum chemical shift was 2.05 ppm, whereas at a lower pH values the phosphorous chemical shift reduced, to 0.34 at pH 5.9. The flowrate and the 31P-NMR chemical shift correlated positively (r = 0.71; p < 0.05). The ICDAS score correlated negatively with the 31P-NMR chemical shift (r = 0.43; p < 0.05).

    Conclusions: This parotid saliva 31P-NMR study has shown that different phosphate states exist within saliva, which significantly influence its inorganic chemical behaviour, and therefore its cariostatic activity.

    Original languageEnglish
    Article number104651
    Pages (from-to)1-6
    Number of pages6
    JournalArchives of Oral Biology
    Volume111
    Early online date7 Jan 2020
    DOIs
    Publication statusE-pub ahead of print - 7 Jan 2020

    Fingerprint

    Dental Caries
    Saliva
    Magnetic Resonance Spectroscopy
    Inorganic Chemicals
    Phosphates
    Waxes
    Dental Enamel
    Paraffin
    Phosphorus
    Minerals
    Healthy Volunteers
    Salts
    Ions

    Keywords

    • P-NMR
    • Dental caries
    • Minimally invasive dentistry
    • Saliva

    Cite this

    Rovera, Angela ; Rovera, Guido ; Alzahrani, Ali ; Hector, Mark ; Anderson, Paul. / Correlation between parotid saliva composition and dental caries using 31P-NMR and ICDAS score. In: Archives of Oral Biology. 2020 ; Vol. 111. pp. 1-6.
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    title = "Correlation between parotid saliva composition and dental caries using 31P-NMR and ICDAS score",
    abstract = "Objectives: The preservation of enamel mineral is influenced by the supersaturation of salivary secretions with respect to calcium phosphate salts. The aim was to measure the chemical environmental state of phosphate ions in a subject's parotid saliva, and to correlate this with their dental caries score, by means of 31P-Nuclear Magnetic Resonance Spectroscopy (31P-NMR) and the International Caries Detection and Assessment System (ICDAS).Design: Unilateral paraffin wax stimulated parotid saliva samples were collected from 21 healthy adult subjects using a Lashley cup. The flowrate was recorded during collection. Clinical caries scores of each subject were classified using the ICDAS score. The pH was recorded for each saliva sample. 31P-NMR spectra of each saliva sample were obtained to determine the phosphorus chemical environment. All the collected data were analysed by Pearson's correlation.Results: Parotid saliva flow rates were in the range from 0.07 to 0.56 ml/min. The pH varied from 5.9 to 7.6. Each 31P-NMR spectrum showed a single broad line with a chemical shift between 0.07 and 2.38 ppm. At neutral pH the maximum chemical shift was 2.05 ppm, whereas at a lower pH values the phosphorous chemical shift reduced, to 0.34 at pH 5.9. The flowrate and the 31P-NMR chemical shift correlated positively (r = 0.71; p < 0.05). The ICDAS score correlated negatively with the 31P-NMR chemical shift (r = 0.43; p < 0.05).Conclusions: This parotid saliva 31P-NMR study has shown that different phosphate states exist within saliva, which significantly influence its inorganic chemical behaviour, and therefore its cariostatic activity.",
    keywords = "P-NMR, Dental caries, Minimally invasive dentistry, Saliva",
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    Correlation between parotid saliva composition and dental caries using 31P-NMR and ICDAS score. / Rovera, Angela (Lead / Corresponding author); Rovera, Guido; Alzahrani, Ali; Hector, Mark; Anderson, Paul.

    In: Archives of Oral Biology, Vol. 111, 104651, 03.2020, p. 1-6.

    Research output: Contribution to journalArticle

    TY - JOUR

    T1 - Correlation between parotid saliva composition and dental caries using 31P-NMR and ICDAS score

    AU - Rovera, Angela

    AU - Rovera, Guido

    AU - Alzahrani, Ali

    AU - Hector, Mark

    AU - Anderson, Paul

    N1 - No Funding

    PY - 2020/1/7

    Y1 - 2020/1/7

    N2 - Objectives: The preservation of enamel mineral is influenced by the supersaturation of salivary secretions with respect to calcium phosphate salts. The aim was to measure the chemical environmental state of phosphate ions in a subject's parotid saliva, and to correlate this with their dental caries score, by means of 31P-Nuclear Magnetic Resonance Spectroscopy (31P-NMR) and the International Caries Detection and Assessment System (ICDAS).Design: Unilateral paraffin wax stimulated parotid saliva samples were collected from 21 healthy adult subjects using a Lashley cup. The flowrate was recorded during collection. Clinical caries scores of each subject were classified using the ICDAS score. The pH was recorded for each saliva sample. 31P-NMR spectra of each saliva sample were obtained to determine the phosphorus chemical environment. All the collected data were analysed by Pearson's correlation.Results: Parotid saliva flow rates were in the range from 0.07 to 0.56 ml/min. The pH varied from 5.9 to 7.6. Each 31P-NMR spectrum showed a single broad line with a chemical shift between 0.07 and 2.38 ppm. At neutral pH the maximum chemical shift was 2.05 ppm, whereas at a lower pH values the phosphorous chemical shift reduced, to 0.34 at pH 5.9. The flowrate and the 31P-NMR chemical shift correlated positively (r = 0.71; p < 0.05). The ICDAS score correlated negatively with the 31P-NMR chemical shift (r = 0.43; p < 0.05).Conclusions: This parotid saliva 31P-NMR study has shown that different phosphate states exist within saliva, which significantly influence its inorganic chemical behaviour, and therefore its cariostatic activity.

    AB - Objectives: The preservation of enamel mineral is influenced by the supersaturation of salivary secretions with respect to calcium phosphate salts. The aim was to measure the chemical environmental state of phosphate ions in a subject's parotid saliva, and to correlate this with their dental caries score, by means of 31P-Nuclear Magnetic Resonance Spectroscopy (31P-NMR) and the International Caries Detection and Assessment System (ICDAS).Design: Unilateral paraffin wax stimulated parotid saliva samples were collected from 21 healthy adult subjects using a Lashley cup. The flowrate was recorded during collection. Clinical caries scores of each subject were classified using the ICDAS score. The pH was recorded for each saliva sample. 31P-NMR spectra of each saliva sample were obtained to determine the phosphorus chemical environment. All the collected data were analysed by Pearson's correlation.Results: Parotid saliva flow rates were in the range from 0.07 to 0.56 ml/min. The pH varied from 5.9 to 7.6. Each 31P-NMR spectrum showed a single broad line with a chemical shift between 0.07 and 2.38 ppm. At neutral pH the maximum chemical shift was 2.05 ppm, whereas at a lower pH values the phosphorous chemical shift reduced, to 0.34 at pH 5.9. The flowrate and the 31P-NMR chemical shift correlated positively (r = 0.71; p < 0.05). The ICDAS score correlated negatively with the 31P-NMR chemical shift (r = 0.43; p < 0.05).Conclusions: This parotid saliva 31P-NMR study has shown that different phosphate states exist within saliva, which significantly influence its inorganic chemical behaviour, and therefore its cariostatic activity.

    KW - P-NMR

    KW - Dental caries

    KW - Minimally invasive dentistry

    KW - Saliva

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