CRISPR/Cas9 Editing of the Plasmodium falciparum Genome

Sophie Adjalley, Marcus Chee San Lee (Lead / Corresponding author)

Research output: Chapter in Book/Report/Conference proceedingChapter

8 Citations (Scopus)

Abstract

The ability to interrogate gene function in Plasmodium parasites has been greatly enhanced by the advent of CRISPR/Cas9 systems. The breadth of genome manipulations ranges from single point mutations to large multigene deletions, however many of the technical considerations for designing CRISPR-based experiments are common to any editing approach. This review will discuss protocols for vector construction and donor design for genome editing P. falciparum, including pitfalls, variables, and validation methods.

Original languageEnglish
Title of host publicationMalaria Immunology
EditorsAnja Tatiana Ramstedt Jensen, Lars Hviid
Place of PublicationNew York
PublisherHumana Press Inc.
Pages221-239
Number of pages19
Edition1
ISBN (Electronic)9781071621899
ISBN (Print)9781071621882, 9781071621912
DOIs
Publication statusPublished - 2022

Publication series

NameMethods in Molecular Biology
Volume2470
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Cas9
  • CRISPR
  • DNA repair
  • Donor template
  • Malaria
  • P. falciparum genome editing

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Fingerprint

Dive into the research topics of 'CRISPR/Cas9 Editing of the Plasmodium falciparum Genome'. Together they form a unique fingerprint.

Cite this