@inbook{226f72e1ece84b569c1b4ee01eb929e2,
title = "CRISPR/Cas9 Editing of the Plasmodium falciparum Genome",
abstract = "The ability to interrogate gene function in Plasmodium parasites has been greatly enhanced by the advent of CRISPR/Cas9 systems. The breadth of genome manipulations ranges from single point mutations to large multigene deletions, however many of the technical considerations for designing CRISPR-based experiments are common to any editing approach. This review will discuss protocols for vector construction and donor design for genome editing P. falciparum, including pitfalls, variables, and validation methods.",
keywords = "Cas9, CRISPR, DNA repair, Donor template, Malaria, P. falciparum genome editing",
author = "Sophie Adjalley and Lee, {Marcus Chee San}",
note = "Copyright: {\textcopyright} 2022, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2022",
doi = "10.1007/978-1-0716-2189-9_17",
language = "English",
isbn = "9781071621882",
series = "Methods in Molecular Biology",
publisher = "Humana Press",
pages = "221--239",
editor = "{Ramstedt Jensen}, {Anja Tatiana} and Lars Hviid",
booktitle = "Malaria Immunology",
address = "United States",
edition = "1",
}
