TY - JOUR
T1 - Crossing Phylums
T2 - Butterfly Wing as a Natural Perfusable Three-Dimensional (3D) Bioconstruct for Bone Tissue Engineering
AU - Mostofi, Fatemeh
AU - Mostofi, Marzieh
AU - Niroomand, Behnaz
AU - Hosseini, Saadi
AU - Alipour, Atefeh
AU - Homaeigohar, Shahin
AU - Mohammadi, Javad
AU - Shokrgozar, Mohammad Ali
AU - Shahsavarani, Hosein
N1 - Funding Information:
We are grateful for the valuable assistance of the members of the Laboratory of Reconstructive Medicine and Biomedical Innovations, especially Abbas Rahimi, in their kind scientific discussion.
Publisher Copyright:
© 2022 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2022/6/1
Y1 - 2022/6/1
N2 - Despite the advent of promising technologies in tissue engineering, finding a biomimetic 3D bio-construct capable of enhancing cell attachment, maintenance, and function is still a challenge in producing tailorable scaffolds for bone regeneration. Here, osteostimulatory effects of the butterfly wings as a naturally porous and non-toxic chitinous scaffold on mesenchymal stromal cells are assessed. The topographical characterization of the butterfly wings implied their ability to mimic bone tissue microenvironment, whereas their regenerative potential was validated after a 14-day cell culture. In vivo analysis showed that the scaffold induced no major inflammatory response in Wistar rats. Topographical features of the bioconstruct upregulated the osteogenic genes, including COL1A1, ALP, BGLAP, SPP1, SP7, and AML3 in differentiated cells compared to the cells cultured in the culture plate. However, butterfly wings were shown to provide a biomimetic microstructure and proper bone regenerative capacity through a unique combination of various structural and material properties. Therefore, this novel platform can be confidently recommended for bone tissue engineering applications.
AB - Despite the advent of promising technologies in tissue engineering, finding a biomimetic 3D bio-construct capable of enhancing cell attachment, maintenance, and function is still a challenge in producing tailorable scaffolds for bone regeneration. Here, osteostimulatory effects of the butterfly wings as a naturally porous and non-toxic chitinous scaffold on mesenchymal stromal cells are assessed. The topographical characterization of the butterfly wings implied their ability to mimic bone tissue microenvironment, whereas their regenerative potential was validated after a 14-day cell culture. In vivo analysis showed that the scaffold induced no major inflammatory response in Wistar rats. Topographical features of the bioconstruct upregulated the osteogenic genes, including COL1A1, ALP, BGLAP, SPP1, SP7, and AML3 in differentiated cells compared to the cells cultured in the culture plate. However, butterfly wings were shown to provide a biomimetic microstructure and proper bone regenerative capacity through a unique combination of various structural and material properties. Therefore, this novel platform can be confidently recommended for bone tissue engineering applications.
KW - butterfly wings
KW - chitin-based scaffold
KW - tissue engineering
KW - osteoblasts
KW - mesenchymal stromal cells
UR - http://www.scopus.com/inward/record.url?scp=85131797914&partnerID=8YFLogxK
U2 - 10.3390/jfb13020068
DO - 10.3390/jfb13020068
M3 - Article
C2 - 35735923
SN - 2079-4983
VL - 13
JO - Journal of Functional Biomaterials
JF - Journal of Functional Biomaterials
IS - 2
M1 - 68
ER -