Crystal structure of the Varkud satellite ribozyme

Nikolai B. Suslov, Saurja Dasgupta, Hao Huang, James R. Fuller, David M J Lilley, Phoebe A. Rice, Joseph A. Piccirilli

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

The Varkud satellite (VS) ribozyme mediates rolling-circle replication of a plasmid found in the Neurospora mitochondrion. We report crystal structures of this ribozyme from Neurospora intermedia at 3.1 Å resolution, which revealed an intertwined dimer formed by an exchange of substrate helices. In each protomer, an arrangement of three-way helical junctions organizes seven helices into a global fold that creates a docking site for the substrate helix of the other protomer, resulting in the formation of two active sites in trans. This mode of RNAâ 'RNA association resembles the process of domain swapping in proteins and has implications for RNA regulation and evolution. Within each active site, adenine and guanine nucleobases abut the scissile phosphate, poised to serve direct roles in catalysis. Similarities to the active sites of the hairpin and hammerhead ribozymes highlight the functional importance of active-site features, underscore the ability of RNA to access functional architectures from distant regions of sequence space, and suggest convergent evolution.

Original languageEnglish
Pages (from-to)840-846
Number of pages7
JournalNature Chemical Biology
Volume11
Early online date28 Sep 2015
DOIs
Publication statusPublished - Nov 2015

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Catalytic Domain
RNA
Neurospora
Protein Subunits
Catalytic RNA
Guanine
Adenine
Catalysis
Mitochondria
Plasmids
Phosphates
varkud satellite ribozyme
Proteins

Keywords

  • Ribozymes
  • X-ray crysallography

Cite this

Suslov, N. B., Dasgupta, S., Huang, H., Fuller, J. R., Lilley, D. M. J., Rice, P. A., & Piccirilli, J. A. (2015). Crystal structure of the Varkud satellite ribozyme. Nature Chemical Biology, 11, 840-846. https://doi.org/10.1038/nchembio.1929
Suslov, Nikolai B. ; Dasgupta, Saurja ; Huang, Hao ; Fuller, James R. ; Lilley, David M J ; Rice, Phoebe A. ; Piccirilli, Joseph A. / Crystal structure of the Varkud satellite ribozyme. In: Nature Chemical Biology. 2015 ; Vol. 11. pp. 840-846.
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Suslov, NB, Dasgupta, S, Huang, H, Fuller, JR, Lilley, DMJ, Rice, PA & Piccirilli, JA 2015, 'Crystal structure of the Varkud satellite ribozyme', Nature Chemical Biology, vol. 11, pp. 840-846. https://doi.org/10.1038/nchembio.1929

Crystal structure of the Varkud satellite ribozyme. / Suslov, Nikolai B.; Dasgupta, Saurja; Huang, Hao; Fuller, James R.; Lilley, David M J; Rice, Phoebe A.; Piccirilli, Joseph A.

In: Nature Chemical Biology, Vol. 11, 11.2015, p. 840-846.

Research output: Contribution to journalArticle

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Suslov NB, Dasgupta S, Huang H, Fuller JR, Lilley DMJ, Rice PA et al. Crystal structure of the Varkud satellite ribozyme. Nature Chemical Biology. 2015 Nov;11:840-846. https://doi.org/10.1038/nchembio.1929