Crystal structures of IspF from Plasmodium falciparum and Burkholderia cenocepacia: comparisons inform antimicrobial drug target assessment

Patrick E. F. O'Rourke, Justyna Kalinowska-Tłuścik, Paul K. Fyfe, Alice Dawson, William N. Hunter (Lead / Corresponding author)

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

2C-methyl-D-erythritol-2,4-cyclodiphosphate synthase (IspF) catalyzes the conversion of 4-diphosphocytidyl-2C-methyl-D-erythritol-2-phosphate to 2C-methyl-D-erythritol-2,4-cyclodiphosphate and cytidine monophosphate in production of isoprenoid-precursors via the methylerythritol phosphate biosynthetic pathway. IspF is found in the protozoan Plasmodium falciparum, a parasite that causes cerebral malaria, as well as in many Gram-negative bacteria such as Burkholderia cenocepacia. IspF represents a potential target for development of broad-spectrum antimicrobial drugs since it is proven or inferred as essential in these pathogens and absent from mammals. Structural studies of IspF from these two important yet distinct pathogens, and comparisons with orthologues have been carried out to generate reagents, to support and inform a structure-based approach to early stage drug discovery.
Original languageEnglish
Article number1
Number of pages12
JournalBMC Structural Biology
Volume14
DOIs
Publication statusPublished - 10 Jan 2014

Keywords

  • Amino Acid Sequence
  • Burkholderia cenocepacia
  • Catalytic Domain
  • Citric Acid
  • Crystallography, X-Ray
  • Erythritol
  • Models, Molecular
  • Phosphorus-Oxygen Lyases
  • Plasmodium falciparum
  • Protein Conformation
  • Protein Structure, Secondary
  • Recombinant Proteins
  • Sequence Alignment
  • Substrate Specificity
  • Sugar Phosphates
  • Zinc

Fingerprint Dive into the research topics of 'Crystal structures of IspF from <em>Plasmodium falciparum</em> and <em>Burkholderia cenocepacia</em>: comparisons inform antimicrobial drug target assessment'. Together they form a unique fingerprint.

Cite this