Cultivation of Heligmosomoides polygyrus: An immunomodulatory nematode parasite and its secreted products

Chris J. C. Johnston, Elaine Robertson, Yvonne Harcus, John R. Grainger, Gillian Coakley, Danielle J. Smyth, Henry J. McSorley, Rick Maizels (Lead / Corresponding author)

Research output: Contribution to journalArticlepeer-review

35 Citations (Scopus)
44 Downloads (Pure)

Abstract

Heligmosomoides polygyrus (formerly known as Nematospiroides dubius, and also referred to by some as H. bakeri) is a gastrointestinal helminth that employs multiple immunomodulatory mechanisms to establish chronic infection in mice and closely resembles prevalent human helminth infections. H. polygyrus has been studied extensively in the field of helminth-derived immune regulation and has been found to potently suppress experimental models of allergy and autoimmunity (both with active infection and isolated secreted products). The protocol described in this paper outlines management of the H. polygyrus life cycle for consistent production of L3 larvae, recovery of adult parasites, and collection of their excretory-secretory products (HES).

Original languageEnglish
Article numbere52412
Pages (from-to)1-10
Number of pages10
JournalJournal of Visualized Experiments
Volume98
DOIs
Publication statusPublished - 6 Apr 2015

Keywords

  • Excretory-Secretory products
  • Heligmosomoides polygyrus
  • Helminth
  • Immunology
  • Infection
  • Issue 98
  • Life cycle
  • Mouse

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