Current and future research at SCRI on the molecular genetics of the interactions between potato and its major pathogens

Paul R.J. Birch, Vivian Blok, Mark Phillips, John T. Jones, Helen E. Stewart, James M. Duncan, Glenn J. Bryan, Robbie Waugh, Gary D. Lyon, Stuart MacFarlane, Anna O. Avrova, Stephen C. Whisson, Ian K. Toth

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Little is known about biochemical or signalling pathways in potato that are involved in resistance to pathogens, whether mediated by resistance genes or as a component of 'field' or 'durable' resistance. Moreover, there is even less information comparing resistance in leaf tissue with that in root. Knowledge from such comparisons could be crucial in developing broad-range plant disease resistance strategies. Research at the Scottish Crop Research Institute (SCRI) has focused on discovery of plant genes up-regulated during resistant and susceptible interactions between potato and the foliar pathogen Phytophthora infestans, the stem and tuber pathogen Erwinia carotovora and the root pathogens Globodera pallida and G. rostochiensis (potato cyst nematodes or PCN). These pathogens are amongst the most economically devastating for potato, the world's fourth major crop. Gene discovery at SCRI, through genetic mapping of key resistance-associated loci, and through transcriptional profiling of resistance pathways, is geared to meet the following objectives: to identify receptors responsible for triggering durable disease resistance, to identify common and distinct pathogen response pathways in root and leaf tissues, to compare resistance mechanisms in different pathosystems, to identify key, general, rapidly activated pathogen response promoters, and to characterise the functions of genes activated in resistance responses in potato. We also seek to answer fundamental questions about the pathogens themselves, such as: What genes are required for successful infection? What is the nature of avirulence genes? What are the molecular bases of host-specificity in these pathogens? To tackle these questions we have adopted genomics approaches. For P. infestans, a framework of integrated genetic, physical and transcriptional maps is being constructed to facilitate rapid discovery of genes involved in the interaction with the host plant. With potato cyst nematodes the focus has been on expressed sequences and defining their function through localisation and biochemical characterisation. More recently, RNAi is being developed to aid in defining function. In the case of Erwinia carotovora subsp. atroseptica, and the closely related E. chrysanthemi, full genome sequencing projects for these organisms are nearing completion, and resources have been developed for post-genomic analyses, including transcriptional and proteomic profiling and a transposon mutation grid to rapidly isolate mutants in any given gene.

Original languageEnglish
Title of host publicationXXVI International Horticultural Congress
Subtitle of host publicationPotatoes, Healthy Food for Humanity: International Developments in Breeding, Production, Protection and Utilizaton
PublisherInternational Society for Horticultural Science
Pages95-115
Number of pages21
ISBN (Print)9789066053601
DOIs
Publication statusPublished - 1 Jan 2003

Publication series

NameActa Horticulturae
Volume619
ISSN (Print)0567-7572

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molecular genetics
potatoes
pathogens
crops
genes
cyst nematodes
Phytophthora infestans
disease resistance
Erwinia chrysanthemi
Pectobacterium carotovorum subsp. atrosepticum
Globodera pallida
genomics
Pectobacterium carotovorum
Globodera rostochiensis
gene interaction
host specificity
plant diseases and disorders
resistance mechanisms
transposons
proteomics

Cite this

Birch, P. R. J., Blok, V., Phillips, M., Jones, J. T., Stewart, H. E., Duncan, J. M., ... Toth, I. K. (2003). Current and future research at SCRI on the molecular genetics of the interactions between potato and its major pathogens. In XXVI International Horticultural Congress: Potatoes, Healthy Food for Humanity: International Developments in Breeding, Production, Protection and Utilizaton (pp. 95-115). (Acta Horticulturae; Vol. 619). International Society for Horticultural Science . https://doi.org/10.17660/ActaHortic.2003.619.11
Birch, Paul R.J. ; Blok, Vivian ; Phillips, Mark ; Jones, John T. ; Stewart, Helen E. ; Duncan, James M. ; Bryan, Glenn J. ; Waugh, Robbie ; Lyon, Gary D. ; MacFarlane, Stuart ; Avrova, Anna O. ; Whisson, Stephen C. ; Toth, Ian K. / Current and future research at SCRI on the molecular genetics of the interactions between potato and its major pathogens. XXVI International Horticultural Congress: Potatoes, Healthy Food for Humanity: International Developments in Breeding, Production, Protection and Utilizaton. International Society for Horticultural Science , 2003. pp. 95-115 (Acta Horticulturae).
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abstract = "Little is known about biochemical or signalling pathways in potato that are involved in resistance to pathogens, whether mediated by resistance genes or as a component of 'field' or 'durable' resistance. Moreover, there is even less information comparing resistance in leaf tissue with that in root. Knowledge from such comparisons could be crucial in developing broad-range plant disease resistance strategies. Research at the Scottish Crop Research Institute (SCRI) has focused on discovery of plant genes up-regulated during resistant and susceptible interactions between potato and the foliar pathogen Phytophthora infestans, the stem and tuber pathogen Erwinia carotovora and the root pathogens Globodera pallida and G. rostochiensis (potato cyst nematodes or PCN). These pathogens are amongst the most economically devastating for potato, the world's fourth major crop. Gene discovery at SCRI, through genetic mapping of key resistance-associated loci, and through transcriptional profiling of resistance pathways, is geared to meet the following objectives: to identify receptors responsible for triggering durable disease resistance, to identify common and distinct pathogen response pathways in root and leaf tissues, to compare resistance mechanisms in different pathosystems, to identify key, general, rapidly activated pathogen response promoters, and to characterise the functions of genes activated in resistance responses in potato. We also seek to answer fundamental questions about the pathogens themselves, such as: What genes are required for successful infection? What is the nature of avirulence genes? What are the molecular bases of host-specificity in these pathogens? To tackle these questions we have adopted genomics approaches. For P. infestans, a framework of integrated genetic, physical and transcriptional maps is being constructed to facilitate rapid discovery of genes involved in the interaction with the host plant. With potato cyst nematodes the focus has been on expressed sequences and defining their function through localisation and biochemical characterisation. More recently, RNAi is being developed to aid in defining function. In the case of Erwinia carotovora subsp. atroseptica, and the closely related E. chrysanthemi, full genome sequencing projects for these organisms are nearing completion, and resources have been developed for post-genomic analyses, including transcriptional and proteomic profiling and a transposon mutation grid to rapidly isolate mutants in any given gene.",
author = "Birch, {Paul R.J.} and Vivian Blok and Mark Phillips and Jones, {John T.} and Stewart, {Helen E.} and Duncan, {James M.} and Bryan, {Glenn J.} and Robbie Waugh and Lyon, {Gary D.} and Stuart MacFarlane and Avrova, {Anna O.} and Whisson, {Stephen C.} and Toth, {Ian K.}",
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Birch, PRJ, Blok, V, Phillips, M, Jones, JT, Stewart, HE, Duncan, JM, Bryan, GJ, Waugh, R, Lyon, GD, MacFarlane, S, Avrova, AO, Whisson, SC & Toth, IK 2003, Current and future research at SCRI on the molecular genetics of the interactions between potato and its major pathogens. in XXVI International Horticultural Congress: Potatoes, Healthy Food for Humanity: International Developments in Breeding, Production, Protection and Utilizaton. Acta Horticulturae, vol. 619, International Society for Horticultural Science , pp. 95-115. https://doi.org/10.17660/ActaHortic.2003.619.11

Current and future research at SCRI on the molecular genetics of the interactions between potato and its major pathogens. / Birch, Paul R.J.; Blok, Vivian; Phillips, Mark; Jones, John T.; Stewart, Helen E.; Duncan, James M.; Bryan, Glenn J.; Waugh, Robbie; Lyon, Gary D.; MacFarlane, Stuart; Avrova, Anna O.; Whisson, Stephen C.; Toth, Ian K.

XXVI International Horticultural Congress: Potatoes, Healthy Food for Humanity: International Developments in Breeding, Production, Protection and Utilizaton. International Society for Horticultural Science , 2003. p. 95-115 (Acta Horticulturae; Vol. 619).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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Birch PRJ, Blok V, Phillips M, Jones JT, Stewart HE, Duncan JM et al. Current and future research at SCRI on the molecular genetics of the interactions between potato and its major pathogens. In XXVI International Horticultural Congress: Potatoes, Healthy Food for Humanity: International Developments in Breeding, Production, Protection and Utilizaton. International Society for Horticultural Science . 2003. p. 95-115. (Acta Horticulturae). https://doi.org/10.17660/ActaHortic.2003.619.11