@article{66334a16c5d1488da49af144d74e3b00,
title = "Cutting back malaria: CRISPR/Cas9 genome editing of Plasmodium",
abstract = "CRISPR/Cas9 approaches are revolutionizing our ability to perform functional genomics across a wide range of organisms, including the Plasmodium parasites that cause malaria. The ability to deliver single point mutations, epitope tags and gene deletions at increased speed and scale is enabling our understanding of the biology of these complex parasites, and pointing to potential new therapeutic targets. In this review, we describe some of the biological and technical considerations for designing CRISPR-based experiments, and discuss potential future developments that broaden the applications for CRISPR/Cas9 interrogation of the malaria parasite genome.",
keywords = "apicomplexan, CRISPR/Cas9, gene regulation, genome editing, malaria, parasite, Plasmodium",
author = "Lee, {Marcus C.S.} and Lindner, {Scott E.} and Lopez-Rubio, {Jose Juan} and Manuel Llin{\'a}s",
note = "Funding Information: We are grateful that early on in our efforts (December 2016) to foster discussion among the parasitology groups using CRISPR/Cas9-based methods, we were fortunate to receive generous support from Wellcome and the Burroughs Wellcome Fund to hold a workshop at the Wellcome Sanger Institute entitled: CRISPR Approaches for Apicomplexans. Lee is supported by a core grant from Wellcome (206194), Llin{\'a}s by National Institute of Allergy and Infectious Diseases (NIAID) R01AI125565, Lindner by NIAID R21AI130692 and Lopez-Rubio by ATIP-AVENIR, ParaFrap (ANR-11- LABX0024) and Fondation pour la Recherche M{\'e}dicale (FRM) (DEQ20180339199). We would like to note two recent papers that would be of interest to readers: Bryant et al. (2019) PMID: 31006600, and Xu et al. (2019) PMID 31202685. Publisher Copyright: {\textcopyright} 2019 The Author(s). Published by Oxford University Press.",
year = "2019",
month = sep,
doi = "10.1093/bfgp/elz012",
language = "English",
volume = "18",
pages = "281--289",
journal = "Briefings in Functional Genomics",
issn = "2041-2649",
publisher = "Oxford University Press",
number = "5",
}
