Abstract
Cyclic di-GMP, an important prokaryote second messenger is used by the eukaryote Dictyostelium discoideum as a secreted signal to trigger stalk formation in fruiting bodies. Cyclic di-GMP is synthesized by a prokaryote-type diguanylate cyclase DgcA, but its mode of action was unknown. Transcriptional profiling yielded several target genes for cyclic di-GMP, which were tested for cyclic di- GMP induced expression in mutants with similar phenotypes as dgca-. A mutant with reduced PKA activity showed defective cyclic di-GMP induced stalk gene expression. Cyclic di-GMP increased cAMP levels in wild-type cells, but not in a mutant that lacked adenylate cyclase A (ACA) activity in slugs. This mutant also did not show cyclic di-GMP induced stalk gene expression. The stalk-less dgca- mutant regained its stalk by expression of a light-activated adenylate cyclase from the ACA promoter and exposure to light, indicating that cAMP is the intermediate for cyclic di-GMP in normal development. ACA is expressed at the tip of emerging fruiting bodies, where it produces the cAMP pulses that organize morphogenetic movement. The tip is also the site where stalk differentiation initiates. Our finding that cyclic di- GMP acts on tip-expressed ACA explains why the Dictyostelium stalk is always formed at the morphogenetic organizer.
Original language | English |
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Title of host publication | Microbial Cyclic Di-Nucleotide Signaling |
Editors | Shan-Ho Chou, Nicolas Guiliani, Vincent T. Lee, Ute Romling |
Place of Publication | Switzerland |
Publisher | Springer International Publishing |
Chapter | 32 |
Pages | 563-574 |
Number of pages | 12 |
Edition | 1 |
ISBN (Electronic) | 9783030333089 |
ISBN (Print) | 9783030333072, 9783030333102 |
DOIs | |
Publication status | Published - 5 Mar 2020 |
Keywords
- Adenylate cyclase A
- Cyclic di-GMP
- Dictyostelium discoideum
- Fruiting bodies
- Organizer
- Protein kinase A
- Stalk formation
ASJC Scopus subject areas
- General Immunology and Microbiology
- General Biochemistry,Genetics and Molecular Biology
- General Medicine